1qx7
From Proteopedia
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[[Image:1qx7.gif|left|200px]] | [[Image:1qx7.gif|left|200px]] | ||
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'''Crystal structure of apoCaM bound to the gating domain of small conductance Ca2+-activated potassium channel''' | '''Crystal structure of apoCaM bound to the gating domain of small conductance Ca2+-activated potassium channel''' | ||
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[[Category: Miller, M C.]] | [[Category: Miller, M C.]] | ||
[[Category: Schumacher, M A.]] | [[Category: Schumacher, M A.]] | ||
| - | [[Category: | + | [[Category: Apocalmodulin]] |
| - | [[Category: | + | [[Category: Ca2+-activated gating]] |
| - | [[Category: | + | [[Category: Cambd]] |
| - | [[Category: | + | [[Category: Functioanl bipartism]] |
| - | [[Category: | + | [[Category: Gating domain]] |
| - | [[Category: | + | [[Category: Sk channel]] |
| - | [[Category: | + | [[Category: Small conductance ca2+ activated k+ channel]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:48:33 2008'' | |
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Revision as of 03:48, 3 May 2008
Crystal structure of apoCaM bound to the gating domain of small conductance Ca2+-activated potassium channel
Overview
Small conductance Ca2+-activated K+ channels (SK channels) are composed of the pore-forming alpha subunit and calmodulin (CaM). CaM binds to a region of the alpha subunit called the CaM binding domain (CaMBD), located intracellular and immediately C-terminal to the inner helix gate, in either the presence or absence of Ca2+. SK gating occurs when Ca2+ binds the N lobe of CaM thereby transmitting the signal to the attached inner helix gate to open. Here we present crystal structures of apoCaM and apoCaM/SK2 CaMBD complex. Several apoCaM crystal forms with multiple (12) packing environments reveal the same EF hand domain-swapped dimer providing potentially new insight into CaM regulation. The apoCaM/SK2 CaMBD structure, combined with our Ca2+/CaM/CaMBD structure suggests that Ca2+ binding induces folding and dimerization of the CaMBD, which causes large CaMBD-CaM C lobe conformational changes, including a >90 degrees rotation of the region of the CaMBD directly connected to the gate.
About this Structure
1QX7 is a Protein complex structure of sequences from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
Crystal structures of apocalmodulin and an apocalmodulin/SK potassium channel gating domain complex., Schumacher MA, Crum M, Miller MC, Structure. 2004 May;12(5):849-60. PMID:15130477 Page seeded by OCA on Sat May 3 06:48:33 2008
