1qxb
From Proteopedia
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[[Image:1qxb.gif|left|200px]] | [[Image:1qxb.gif|left|200px]] | ||
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| - | + | {{STRUCTURE_1qxb| PDB=1qxb | SCENE= }} | |
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'''NMR structure determination of the self complementary DNA Dodecamer CGCGAATT*CGCG in which a ribose is inserted between the 3'-OH of T8 and the 5'-phosphate group of C9''' | '''NMR structure determination of the self complementary DNA Dodecamer CGCGAATT*CGCG in which a ribose is inserted between the 3'-OH of T8 and the 5'-phosphate group of C9''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QXB OCA]. | |
==Reference== | ==Reference== | ||
Cleavage of DNA without loss of genetic information by incorporation of a disaccharide nucleoside., Nauwelaerts K, Vastmans K, Froeyen M, Kempeneers V, Rozenski J, Rosemeyer H, Van Aerschot A, Busson R, Lacey JC, Efimtseva E, Mikhailov S, Lescrinier E, Herdewijn P, Nucleic Acids Res. 2003 Dec 1;31(23):6758-69. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14627809 14627809] | Cleavage of DNA without loss of genetic information by incorporation of a disaccharide nucleoside., Nauwelaerts K, Vastmans K, Froeyen M, Kempeneers V, Rozenski J, Rosemeyer H, Van Aerschot A, Busson R, Lacey JC, Efimtseva E, Mikhailov S, Lescrinier E, Herdewijn P, Nucleic Acids Res. 2003 Dec 1;31(23):6758-69. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14627809 14627809] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Aerschot, A Van.]] | [[Category: Aerschot, A Van.]] | ||
[[Category: Busson, R.]] | [[Category: Busson, R.]] | ||
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[[Category: Rozenski, J.]] | [[Category: Rozenski, J.]] | ||
[[Category: Vastmans, K.]] | [[Category: Vastmans, K.]] | ||
| - | [[Category: | + | [[Category: B-helix]] |
| - | [[Category: | + | [[Category: Backbone modification]] |
| - | [[Category: | + | [[Category: Disaccharide nucleotide]] |
| - | [[Category: | + | [[Category: Ribose]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:48:46 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 03:48, 3 May 2008
NMR structure determination of the self complementary DNA Dodecamer CGCGAATT*CGCG in which a ribose is inserted between the 3'-OH of T8 and the 5'-phosphate group of C9
Overview
A ribose residue inserted between the 3'-OH of one nucleotide and the 5'-phosphate group of the next nucleotide, functions as a site-specific cleavage site within DNA. This extra ribose does not interrupt helix formation and it protects duplex DNA against cleavage by restriction enzymes. Cleavage can be obtained with periodate and all ribose fragments can be removed with sodium hydroxide. As a result of this, an intact natural oligodeoxynucleotide is obtained after ligation reaction, which means that site-specific cleavage and recovering of intact DNA occurs without loss of genetic information.
About this Structure
Full crystallographic information is available from OCA.
Reference
Cleavage of DNA without loss of genetic information by incorporation of a disaccharide nucleoside., Nauwelaerts K, Vastmans K, Froeyen M, Kempeneers V, Rozenski J, Rosemeyer H, Van Aerschot A, Busson R, Lacey JC, Efimtseva E, Mikhailov S, Lescrinier E, Herdewijn P, Nucleic Acids Res. 2003 Dec 1;31(23):6758-69. PMID:14627809 Page seeded by OCA on Sat May 3 06:48:46 2008
