This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


5w3t

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (14:13, 13 March 2024) (edit) (undo)
 
Line 3: Line 3:
<StructureSection load='5w3t' size='340' side='right'caption='[[5w3t]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
<StructureSection load='5w3t' size='340' side='right'caption='[[5w3t]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[5w3t]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_solanacearum"_smith_1896 "bacillus solanacearum" smith 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W3T OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5W3T FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[5w3t]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Ralstonia_solanacearum Ralstonia solanacearum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W3T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5W3T FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IHP:INOSITOL+HEXAKISPHOSPHATE'>IHP</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
-
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5w3x|5w3x]], [[5w40|5w40]], [[5w3y|5w3y]]</td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IHP:INOSITOL+HEXAKISPHOSPHATE'>IHP</scene></td></tr>
-
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">popp, RUN1985_v1_1190012 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=305 "Bacillus solanacearum" Smith 1896])</td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5w3t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w3t OCA], [https://pdbe.org/5w3t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5w3t RCSB], [https://www.ebi.ac.uk/pdbsum/5w3t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5w3t ProSAT]</span></td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5w3t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w3t OCA], [http://pdbe.org/5w3t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w3t RCSB], [http://www.ebi.ac.uk/pdbsum/5w3t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w3t ProSAT]</span></td></tr>
+
</table>
</table>
-
<div style="background-color:#fffaf0;">
+
== Function ==
-
== Publication Abstract from PubMed ==
+
[https://www.uniprot.org/uniprot/A0A0S4VB05_RALSL A0A0S4VB05_RALSL]
-
The Yersinia outer protein J (YopJ) family of bacterial effectors depends on a novel acetyltransferase domain to acetylate signalling proteins from plant and animal hosts. However, the underlying mechanism is unclear. Here, we report the crystal structures of PopP2, a YopJ effector produced by the plant pathogen Ralstonia solanacearum, in complex with inositol hexaphosphate (InsP6), acetyl-coenzyme A (AcCoA) and/or substrate Resistance to Ralstonia solanacearum 1 (RRS1-R)WRKY. PopP2 recognizes the WRKYGQK motif of RRS1-RWRKY to position a targeted lysine in the active site for acetylation. Importantly, the PopP2-RRS1-RWRKY association is allosterically regulated by InsP6 binding, suggesting a previously unidentified role of the eukaryote-specific cofactor in substrate interaction. Furthermore, we provide evidence for the reaction intermediate of PopP2-mediated acetylation, an acetyl-cysteine covalent adduct, lending direct support to the 'ping-pong'-like catalytic mechanism proposed for YopJ effectors. Our study provides critical mechanistic insights into the virulence activity of YopJ class of acetyltransferases.
+
-
 
+
-
Mechanism of host substrate acetylation by a YopJ family effector.,Zhang ZM, Ma KW, Gao L, Hu Z, Schwizer S, Ma W, Song J Nat Plants. 2017 Jul 24;3:17115. doi: 10.1038/nplants.2017.115. PMID:28737762<ref>PMID:28737762</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 5w3t" style="background-color:#fffaf0;"></div>
+
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Bacillus solanacearum smith 1896]]
 
[[Category: Large Structures]]
[[Category: Large Structures]]
-
[[Category: Song, J]]
+
[[Category: Ralstonia solanacearum]]
-
[[Category: Zhang, Z M]]
+
[[Category: Song J]]
-
[[Category: Ip6]]
+
[[Category: Zhang ZM]]
-
[[Category: Popp2]]
+
-
[[Category: Transcription]]
+
-
[[Category: Yopj effector]]
+

Current revision

Crystal structure of PopP2 in complex with IP6

PDB ID 5w3t

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5w3t"
Personal tools