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| | <StructureSection load='5w8s' size='340' side='right'caption='[[5w8s]], [[Resolution|resolution]] 2.10Å' scene=''> | | <StructureSection load='5w8s' size='340' side='right'caption='[[5w8s]], [[Resolution|resolution]] 2.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5w8s]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecobd Ecobd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W8S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W8S FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5w8s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_BL21(DE3) Escherichia coli BL21(DE3)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W8S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5W8S FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LI:LITHIUM+ION'>LI</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5w8x|5w8x]], [[5w8n|5w8n]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LI:LITHIUM+ION'>LI</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lpxB, ECBD_3437 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=469008 ECOBD])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5w8s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w8s OCA], [https://pdbe.org/5w8s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5w8s RCSB], [https://www.ebi.ac.uk/pdbsum/5w8s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5w8s ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lipid-A-disaccharide_synthase Lipid-A-disaccharide synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.182 2.4.1.182] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5w8s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w8s OCA], [http://pdbe.org/5w8s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w8s RCSB], [http://www.ebi.ac.uk/pdbsum/5w8s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w8s ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/A0A140NAT1_ECOBD A0A140NAT1_ECOBD]] Condensation of UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.[HAMAP-Rule:MF_00392][SAAS:SAAS00702254] | + | [https://www.uniprot.org/uniprot/LPXB_ECOLI LPXB_ECOLI] Condensation of UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell. |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | Most Gram-negative bacteria are surrounded by a glycolipid called lipopolysaccharide (LPS), which forms a barrier to hydrophobic toxins and, in pathogenic bacteria, is a virulence factor. During LPS biosynthesis, a membrane-associated glycosyltransferase (LpxB) forms a tetra-acylated disaccharide that is further acylated to form the membrane anchor moiety of LPS. Here we solve the structure of a soluble and catalytically competent LpxB by X-ray crystallography. The structure reveals that LpxB has a glycosyltransferase-B family fold but with a highly intertwined, C-terminally swapped dimer comprising four domains. We identify key catalytic residues with a product, UDP, bound in the active site, as well as clusters of hydrophobic residues that likely mediate productive membrane association or capture of lipidic substrates. These studies provide the basis for rational design of antibiotics targeting a crucial step in LPS biosynthesis.
| + | |
| - | | + | |
| - | Crystal structure of lipid A disaccharide synthase LpxB from Escherichia coli.,Bohl TE, Shi K, Lee JK, Aihara H Nat Commun. 2018 Jan 25;9(1):377. doi: 10.1038/s41467-017-02712-9. PMID:29371662<ref>PMID:29371662</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 5w8s" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Ecobd]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Lipid-A-disaccharide synthase]]
| + | [[Category: Aihara H]] |
| - | [[Category: Aihara, H]] | + | [[Category: Bohl TE]] |
| - | [[Category: Bohl, T E]] | + | [[Category: Lee JK]] |
| - | [[Category: Lee, J K]] | + | [[Category: Shi K]] |
| - | [[Category: Shi, K]] | + | |
| - | [[Category: C-terminal swap]]
| + | |
| - | [[Category: Dimer]]
| + | |
| - | [[Category: Glycosyltransferase b]]
| + | |
| - | [[Category: Lipid a disaccharide synthase]]
| + | |
| - | [[Category: Lipid a synthesis pathway]]
| + | |
| - | [[Category: Lipiopolysaccharide synthesis]]
| + | |
| - | [[Category: Raetz pathway]]
| + | |
| - | [[Category: Rossmann-like]]
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| - | [[Category: Transferase]]
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