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| | <SX load='6b0x' size='340' side='right' viewer='molstar' caption='[[6b0x]], [[Resolution|resolution]] 3.72Å' scene=''> | | <SX load='6b0x' size='340' side='right' viewer='molstar' caption='[[6b0x]], [[Resolution|resolution]] 3.72Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6b0x]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_80_alpha Bacteriophage 80 alpha]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B0X OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6B0X FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6b0x]] is a 14 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_virus_80alpha Staphylococcus virus 80alpha]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B0X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6B0X FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6b23|6b23]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.72Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">orf47 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=53369 Bacteriophage 80 alpha])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6b0x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b0x OCA], [https://pdbe.org/6b0x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6b0x RCSB], [https://www.ebi.ac.uk/pdbsum/6b0x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6b0x ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6b0x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b0x OCA], [http://pdbe.org/6b0x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6b0x RCSB], [http://www.ebi.ac.uk/pdbsum/6b0x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6b0x ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/A4ZFB3_9CAUD A4ZFB3_9CAUD] |
| - | Staphylococcus aureus pathogenicity islands (SaPIs), such as SaPI1, exploit specific helper bacteriophages, like 80alpha, for their high frequency mobilization, a process termed 'molecular piracy'. SaPI1 redirects the helper's assembly pathway to form small capsids that can only accommodate the smaller SaPI1 genome, but not a complete phage genome. SaPI1 encodes two proteins, CpmA and CpmB, that are responsible for this size redirection. We have determined the structures of the 80alpha and SaPI1 procapsids to near-atomic resolution by cryo-electron microscopy, and show that CpmB competes with the 80alpha scaffolding protein (SP) for a binding site on the capsid protein (CP), and works by altering the angle between capsomers. We probed these interactions genetically and identified second-site suppressors of lethal mutations in SP. Our structures show, for the first time, the detailed interactions between SP and CP in a bacteriophage, providing unique insights into macromolecular assembly processes.
| + | |
| - | | + | |
| - | Competing scaffolding proteins determine capsid size during mobilization of Staphylococcus aureus pathogenicity islands.,Dearborn AD, Wall EA, Kizziah JL, Klenow L, Parker LK, Manning KA, Spilman MS, Spear JM, Christie GE, Dokland T Elife. 2017 Oct 6;6. pii: e30822. doi: 10.7554/eLife.30822. PMID:28984245<ref>PMID:28984245</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 6b0x" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </SX> | | </SX> |
| - | [[Category: Bacteriophage 80 alpha]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Dearborn, A D]] | + | [[Category: Staphylococcus virus 80alpha]] |
| - | [[Category: Dokland, T]] | + | [[Category: Dearborn AD]] |
| - | [[Category: Kizziah, J L]] | + | [[Category: Dokland T]] |
| - | [[Category: Hk97-like fold]] | + | [[Category: Kizziah JL]] |
| - | [[Category: Major capsid protein]]
| + | |
| - | [[Category: Procapsid]]
| + | |
| - | [[Category: Scaffolding protein]]
| + | |
| - | [[Category: Virus]]
| + | |
