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| | ==Crystal structure of Chaetomium thermophilum Gle1 CTD-Nup42 GBM complex== | | ==Crystal structure of Chaetomium thermophilum Gle1 CTD-Nup42 GBM complex== |
| - | <StructureSection load='6b4g' size='340' side='right' caption='[[6b4g]], [[Resolution|resolution]] 2.65Å' scene=''> | + | <StructureSection load='6b4g' size='340' side='right'caption='[[6b4g]], [[Resolution|resolution]] 2.65Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6b4g]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Chatd Chatd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B4G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B4G FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6b4g]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum_var._thermophilum_DSM_1495 Chaetomium thermophilum var. thermophilum DSM 1495]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B4G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6B4G FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.648Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GLE1, CTHT_0027940 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=759272 CHATD]), AMO1, CTHT_0020020 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=759272 CHATD])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6b4g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b4g OCA], [http://pdbe.org/6b4g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6b4g RCSB], [http://www.ebi.ac.uk/pdbsum/6b4g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6b4g ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6b4g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b4g OCA], [https://pdbe.org/6b4g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6b4g RCSB], [https://www.ebi.ac.uk/pdbsum/6b4g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6b4g ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/GLE1_CHATD GLE1_CHATD]] Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors.[UniProtKB:Q12315] [[http://www.uniprot.org/uniprot/AMO1_CHATD AMO1_CHATD]] Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. Active directional transport is assured by both, a Phe-Gly (FG) repeat affinity gradient for these transport factors across the NPC and a transport cofactor concentration gradient across the nuclear envelope (GSP1 and GSP2 GTPases associated predominantly with GTP in the nucleus, with GDP in the cytoplasm). AMO1 is specifically important for nuclear protein and mRNA export.[UniProtKB:P49686] | + | [https://www.uniprot.org/uniprot/GLE1_CHATD GLE1_CHATD] Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors.[UniProtKB:Q12315] |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | The nuclear pore complex (NPC) controls the passage of macromolecules between the nucleus and cytoplasm, but how the NPC directly participates in macromolecular transport remains poorly understood. In the final step of mRNA export, the DEAD-box helicase DDX19 is activated by the nucleoporins Gle1, Nup214, and Nup42 to remove Nxf1*Nxt1 from mRNAs. Here, we report crystal structures of Gle1*Nup42 from three organisms that reveal an evolutionarily conserved binding mode. Biochemical reconstitution of the DDX19 ATPase cycle establishes that human DDX19 activation does not require IP6, unlike its fungal homologs, and that Gle1 stability affects DDX19 activation. Mutations linked to motor neuron diseases cause decreased Gle1 thermostability, implicating nucleoporin misfolding as a disease determinant. Crystal structures of human Gle1*Nup42*DDX19 reveal the structural rearrangements in DDX19 from an auto-inhibited to an RNA-binding competent state. Together, our results provide the foundation for further mechanistic analyses of mRNA export in humans.
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| - | Structural and functional analysis of mRNA export regulation by the nuclear pore complex.,Lin DH, Correia AR, Cai SW, Huber FM, Jette CA, Hoelz A Nat Commun. 2018 Jun 13;9(1):2319. doi: 10.1038/s41467-018-04459-3. PMID:29899397<ref>PMID:29899397</ref>
| + | ==See Also== |
| - | | + | *[[Nucleoporin 3D structures|Nucleoporin 3D structures]] |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 6b4g" style="background-color:#fffaf0;"></div>
| + | |
| - | == References == | + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Chatd]] | + | [[Category: Chaetomium thermophilum var. thermophilum DSM 1495]] |
| - | [[Category: Cai, S W]] | + | [[Category: Large Structures]] |
| - | [[Category: Correia, A R]] | + | [[Category: Cai SW]] |
| - | [[Category: Hoelz, A]] | + | [[Category: Correia AR]] |
| - | [[Category: Huber, F M]] | + | [[Category: Hoelz A]] |
| - | [[Category: Jette, C A]]
| + | [[Category: Huber FM]] |
| - | [[Category: Lin, D H]] | + | [[Category: Jette CA]] |
| - | [[Category: Complex]] | + | [[Category: Lin DH]] |
| - | [[Category: Dead-box helicase]] | + | |
| - | [[Category: Mrna export]]
| + | |
| - | [[Category: Nuclear pore complex]]
| + | |
| - | [[Category: Transport protein]]
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