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| | <StructureSection load='6bbh' size='340' side='right'caption='[[6bbh]], [[Resolution|resolution]] 6.10Å' scene=''> | | <StructureSection load='6bbh' size='340' side='right'caption='[[6bbh]], [[Resolution|resolution]] 6.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6bbh]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BBH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BBH FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6bbh]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BBH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6BBH FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">olf186-F, CRACM1, olf186, CG11430 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 6.1Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bbh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bbh OCA], [http://pdbe.org/6bbh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bbh RCSB], [http://www.ebi.ac.uk/pdbsum/6bbh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bbh ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6bbh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bbh OCA], [https://pdbe.org/6bbh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6bbh RCSB], [https://www.ebi.ac.uk/pdbsum/6bbh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6bbh ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CRCM1_DROME CRCM1_DROME]] Ca(2+) release-activated Ca(2+) (CRAC) channel subunit which mediates Ca(2+) influx following depletion of intracellular Ca(2+) stores. Regulates transcription factor NFAT nuclear import.<ref>PMID:16582901</ref> <ref>PMID:16645049</ref> | + | [https://www.uniprot.org/uniprot/CRCM1_DROME CRCM1_DROME] Ca(2+) release-activated Ca(2+) (CRAC) channel subunit which mediates Ca(2+) influx following depletion of intracellular Ca(2+) stores. Regulates transcription factor NFAT nuclear import.<ref>PMID:16582901</ref> <ref>PMID:16645049</ref> |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | The store-operated calcium (Ca(2+)) channel Orai governs Ca(2+) influx through the plasma membrane of many non-excitable cells in metazoans. The channel opens in response to the depletion of Ca(2+) stored in the endoplasmic reticulum (ER). Loss- and gain-of-function mutants of Orai cause disease. Our previous work revealed the structure of Orai with a closed pore. Here, using a gain-of-function mutation that constitutively activates the channel, we present an X-ray structure of Drosophila melanogaster Orai in an open conformation. Well-defined electron density maps reveal that the pore is dramatically dilated on its cytosolic side in comparison to the slender closed pore. Cations and anions bind in different regions of the open pore, informing mechanisms for ion permeation and Ca(2+) selectivity. Opening of the pore requires the release of cytosolic latches. Together with additional X-ray structures of an unlatched-but-closed conformation, we propose a sequence for store-operated activation.
| + | |
| - | | + | |
| - | Structures reveal opening of the store-operated calcium channel Orai.,Hou X, Burstein SR, Long SB Elife. 2018 Aug 30;7. pii: 36758. doi: 10.7554/eLife.36758. PMID:30160233<ref>PMID:30160233</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 6bbh" style="background-color:#fffaf0;"></div>
| + | |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Drome]] | + | [[Category: Drosophila melanogaster]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Burstein, S]] | + | [[Category: Burstein S]] |
| - | [[Category: Hou, X]] | + | [[Category: Hou X]] |
| - | [[Category: Long, S B]] | + | [[Category: Long SB]] |
| - | [[Category: Calcium]]
| + | |
| - | [[Category: Crac]]
| + | |
| - | [[Category: Eukaryotic]]
| + | |
| - | [[Category: Ion channel]]
| + | |
| - | [[Category: Membrane protein]]
| + | |
| - | [[Category: Open structure]]
| + | |
| - | [[Category: Soce]]
| + | |
| Structural highlights
Function
CRCM1_DROME Ca(2+) release-activated Ca(2+) (CRAC) channel subunit which mediates Ca(2+) influx following depletion of intracellular Ca(2+) stores. Regulates transcription factor NFAT nuclear import.[1] [2]
References
- ↑ Feske S, Gwack Y, Prakriya M, Srikanth S, Puppel SH, Tanasa B, Hogan PG, Lewis RS, Daly M, Rao A. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature. 2006 May 11;441(7090):179-85. Epub 2006 Apr 2. PMID:16582901 doi:http://dx.doi.org/nature04702
- ↑ Vig M, Peinelt C, Beck A, Koomoa DL, Rabah D, Koblan-Huberson M, Kraft S, Turner H, Fleig A, Penner R, Kinet JP. CRACM1 is a plasma membrane protein essential for store-operated Ca2+ entry. Science. 2006 May 26;312(5777):1220-3. Epub 2006 Apr 27. PMID:16645049 doi:http://dx.doi.org/10.1126/science.1127883
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