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6bk5

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Current revision (14:22, 13 March 2024) (edit) (undo)
 
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<StructureSection load='6bk5' size='340' side='right'caption='[[6bk5]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
<StructureSection load='6bk5' size='340' side='right'caption='[[6bk5]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6bk5]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BK5 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6BK5 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6bk5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Salpingoeca_rosetta Salpingoeca rosetta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BK5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6BK5 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.401&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6bk5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bk5 OCA], [http://pdbe.org/6bk5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bk5 RCSB], [http://www.ebi.ac.uk/pdbsum/6bk5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bk5 ProSAT]</span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6bk5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bk5 OCA], [https://pdbe.org/6bk5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6bk5 RCSB], [https://www.ebi.ac.uk/pdbsum/6bk5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6bk5 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/F2UL73_SALR5 F2UL73_SALR5]
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Cbl proteins are E3 ubiquitin ligases specialized for the regulation of tyrosine kinases by ubiquitylation. Human Cbl proteins are activated by tyrosine phosphorylation, thus setting up a feedback loop whereby the activation of tyrosine kinases triggers their own degradation. Cbl proteins are targeted to their substrates by a phosphotyrosine-binding SH2 domain. Choanoflagellates, unicellular eukaryotes that are closely related to metazoans, also contain Cbl. The tyrosine kinase complement of choanoflagellates is distinct from that of metazoans, and it is unclear if choanoflagellate Cbl is regulated similarly to metazoan Cbl. Here, we performed structure-function studies on Cbl from the choanoflagellate species Salpingoeca rosetta, and found that it undergoes phosphorylation-dependent activation. We show that S. rosetta Cbl can be phosphorylated by S. rosetta Src kinase, and that it can ubiquitylate S. rosetta Src. We also compared the substrate selectivity of human and S. rosetta Cbl by measuring ubiquitylation of Src constructs in which Cbl-recruitment sites are placed in different contexts with respect to the kinase domain. Our results indicate that for both human and S. rosetta Cbl, ubiquitylation depends on proximity and accessibility, rather than being targeted toward specific lysine residues. Our results point to an ancient interplay between phosphotyrosine and ubiquitin signaling in the metazoan lineage. This article is protected by copyright. All rights reserved.
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Phosphorylation Control of the Ubiquitin Ligase Cbl is Conserved in Choanoflagellates.,Amacher JF, Hobbs HT, Cantor AC, Shah L, Rivero MJ, Mulchand SA, Kuriyan J Protein Sci. 2018 Mar 2. doi: 10.1002/pro.3397. PMID:29498112<ref>PMID:29498112</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6bk5" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
*[[Ubiquitin protein ligase 3D structures|Ubiquitin protein ligase 3D structures]]
*[[Ubiquitin protein ligase 3D structures|Ubiquitin protein ligase 3D structures]]
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== References ==
 
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<references/>
 
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Amacher, J F]]
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[[Category: Salpingoeca rosetta]]
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[[Category: Kuriyan, J]]
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[[Category: Amacher JF]]
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[[Category: Cbl]]
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[[Category: Kuriyan J]]
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[[Category: Signaling protein]]
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[[Category: Ubiquitin ligase]]
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Current revision

Inactive choanoflagellate E3 ubiquitin ligase Cbl TKB

PDB ID 6bk5

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