|
|
| Line 3: |
Line 3: |
| | <StructureSection load='6bs8' size='340' side='right'caption='[[6bs8]], [[Resolution|resolution]] 1.95Å' scene=''> | | <StructureSection load='6bs8' size='340' side='right'caption='[[6bs8]], [[Resolution|resolution]] 1.95Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6bs8]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_smegmatis"_trevisan_1889 "bacillus smegmatis" trevisan 1889]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BS8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BS8 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6bs8]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis Mycolicibacterium smegmatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BS8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6BS8 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">dnaB, ERS451418_06678 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1772 "Bacillus smegmatis" Trevisan 1889])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_helicase DNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.12 3.6.4.12] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6bs8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bs8 OCA], [https://pdbe.org/6bs8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6bs8 RCSB], [https://www.ebi.ac.uk/pdbsum/6bs8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6bs8 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bs8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bs8 OCA], [http://pdbe.org/6bs8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bs8 RCSB], [http://www.ebi.ac.uk/pdbsum/6bs8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bs8 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/A0A0D6J385_MYCSM A0A0D6J385_MYCSM]] Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity.[RuleBase:RU362085] | + | [https://www.uniprot.org/uniprot/I7FPC4_MYCS2 I7FPC4_MYCS2] Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity.[ARBA:ARBA00002835][RuleBase:RU362085] |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | Inteins are widespread self-splicing protein elements emerging as potential post-translational environmental sensors. Here, we describe two inteins within one protein, the Mycobacterium smegmatis replicative helicase DnaB. These inteins, DnaBi1 and DnaBi2, have homology to inteins in pathogens, splice with vastly varied rates, and are differentially responsive to environmental stressors. Whereas DnaBi1 splicing is reversibly inhibited by oxidative and nitrosative insults, DnaBi2 is not. Using a reporter that measures splicing in a native intein-containing organism and western blotting, we show that H2O2 inhibits DnaBi1 splicing in M. smegmatis. Intriguingly, upon oxidation, the catalytic cysteine of DnaBi1 forms an intramolecular disulfide bond. We report a crystal structure of the class 3 DnaBi1 intein at 1.95 A, supporting our findings and providing insight into this splicing mechanism. We propose that this cysteine toggle allows DnaBi1 to sense stress, pausing replication to maintain genome integrity, and then allowing splicing immediately when permissive conditions return.
| + | |
| - | | + | |
| - | Mycobacterial DnaB helicase intein as oxidative stress sensor.,Kelley DS, Lennon CW, Li Z, Miller MR, Banavali NK, Li H, Belfort M Nat Commun. 2018 Oct 19;9(1):4363. doi: 10.1038/s41467-018-06554-x. PMID:30341292<ref>PMID:30341292</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 6bs8" style="background-color:#fffaf0;"></div>
| + | |
| | | | |
| | ==See Also== | | ==See Also== |
| | *[[Helicase 3D structures|Helicase 3D structures]] | | *[[Helicase 3D structures|Helicase 3D structures]] |
| - | == References == | |
| - | <references/> | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillus smegmatis trevisan 1889]] | |
| - | [[Category: DNA helicase]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Banavali, N]] | + | [[Category: Mycolicibacterium smegmatis]] |
| - | [[Category: Belfort, M]] | + | [[Category: Banavali N]] |
| - | [[Category: Kelley, D S]] | + | [[Category: Belfort M]] |
| - | [[Category: Li, H]] | + | [[Category: Kelley DS]] |
| - | [[Category: Li, Z]] | + | [[Category: Li H]] |
| - | [[Category: Class 3 intein]]
| + | [[Category: Li Z]] |
| - | [[Category: Dnab]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
