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| | <SX load='6cb1' size='340' side='right' viewer='molstar' caption='[[6cb1]], [[Resolution|resolution]] 4.60Å' scene=''> | | <SX load='6cb1' size='340' side='right' viewer='molstar' caption='[[6cb1]], [[Resolution|resolution]] 4.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6cb1]] is a 40 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_by4741 Saccharomyces cerevisiae by4741]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CB1 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6CB1 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6cb1]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_BY4741 Saccharomyces cerevisiae BY4741]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CB1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6CB1 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.6Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6c0f|6c0f]]</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6cb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cb1 OCA], [https://pdbe.org/6cb1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6cb1 RCSB], [https://www.ebi.ac.uk/pdbsum/6cb1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6cb1 ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA_helicase RNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.13 3.6.4.13] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6cb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cb1 OCA], [http://pdbe.org/6cb1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cb1 RCSB], [http://www.ebi.ac.uk/pdbsum/6cb1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cb1 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/RRP1_YEAST RRP1_YEAST]] Required for 27S rRNA processing to 25S and 5.8S. [[http://www.uniprot.org/uniprot/RL37A_YEAST RL37A_YEAST]] Binds to the 23S rRNA (By similarity). [[http://www.uniprot.org/uniprot/NOP15_YEAST NOP15_YEAST]] Involved in the biogenesis of the 60S ribosomal subunit. Required for pre-rRNA processing and cytokinesis. Associates with the precursors of the 25S and 5.8S rRNAs.<ref>PMID:11583614</ref> <ref>PMID:14657029</ref> [[http://www.uniprot.org/uniprot/NOP16_YEAST NOP16_YEAST]] Involved in the biogenesis of the 60S ribosomal subunit.<ref>PMID:11583614</ref> [[http://www.uniprot.org/uniprot/BRX1_YEAST BRX1_YEAST]] Required for biogenesis of the 60S ribosomal subunit.<ref>PMID:11843177</ref> [[http://www.uniprot.org/uniprot/RLP7_YEAST RLP7_YEAST]] Involved in the biogenesis of the 60S ribosomal subunit. May act as a specificity factor that binds precursor rRNAs and tethers the enzymes that carry out the early 5' to 3' exonucleolytic reactions that generate the mature rRNAs.<ref>PMID:11087857</ref> [[http://www.uniprot.org/uniprot/ERB1_YEAS7 ERB1_YEAS7]] Component of the NOP7 complex, which is required for maturation of the 25S and 5.8S ribosomal RNAs and formation of the 60S ribosome.[HAMAP-Rule:MF_03027] | + | [https://www.uniprot.org/uniprot/RL4A_YEAST RL4A_YEAST] Participates in the regulation of the accumulation of its own mRNA.<ref>PMID:2065661</ref> |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | Early co-transcriptional events of eukaryotic ribosome assembly result in the formation of precursors of the small (40S) and large (60S) ribosomal subunits(1). A multitude of transient assembly factors regulate and chaperone the systematic folding of pre-ribosomal RNA subdomains. However, due to limited structural information, the role of these factors during early nucleolar 60S assembly is not fully understood. Here we have determined cryo-EM reconstructions of the nucleolar pre-60S ribosomal subunit in different conformational states at resolutions up to 3.4 A. These reconstructions reveal how steric hindrance and molecular mimicry are used to prevent both premature folding states and binding of later factors. This is accomplished by the concerted activity of 21 ribosome assembly factors that stabilize and remodel pre-ribosomal RNA and ribosomal proteins. Among these factors, three Brix-domain proteins and their binding partners form a ring-like structure at rRNA domain boundaries to support the architecture of the maturing particle. Mutually exclusive conformations of these pre-60S particles suggest that the formation of the polypeptide exit tunnel is achieved through different folding pathways during subsequent stages of ribosome assembly. These structures rationalize previous genetic and biochemical data and highlight the mechanisms driving eukaryotic ribosome assembly in a unidirectional manner.
| + | |
| - | | + | |
| - | Modular assembly of the nucleolar pre-60S ribosomal subunit.,Sanghai ZA, Miller L, Molloy KR, Barandun J, Hunziker M, Chaker-Margot M, Wang J, Chait BT, Klinge S Nature. 2018 Mar 5. pii: nature26156. doi: 10.1038/nature26156. PMID:29512650<ref>PMID:29512650</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 6cb1" style="background-color:#fffaf0;"></div>
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| | | | |
| | ==See Also== | | ==See Also== |
| | + | *[[Helicase 3D structures|Helicase 3D structures]] |
| | *[[Ribosome 3D structures|Ribosome 3D structures]] | | *[[Ribosome 3D structures|Ribosome 3D structures]] |
| - | *[[Ribosome biogenesis protein|Ribosome biogenesis protein]] | + | *[[Ribosome biogenesis protein 3D structures|Ribosome biogenesis protein 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | </SX> | | </SX> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: RNA helicase]]
| + | [[Category: Saccharomyces cerevisiae BY4741]] |
| - | [[Category: Saccharomyces cerevisiae by4741]] | + | [[Category: Barandun J]] |
| - | [[Category: Barandun, J]] | + | [[Category: Chaker-Margot M]] |
| - | [[Category: Chaker-Margot, M]] | + | [[Category: Hunziker M]] |
| - | [[Category: Hunziker, M]] | + | [[Category: Klinge S]] |
| - | [[Category: Klinge, S]] | + | [[Category: Miller L]] |
| - | [[Category: Miller, L]] | + | [[Category: Sanghai ZA]] |
| - | [[Category: Sanghai, Z A]] | + | |
| - | [[Category: Lsu processome]]
| + | |
| - | [[Category: Pre-60]]
| + | |
| - | [[Category: Ribosome]]
| + | |
| - | [[Category: Ribosome biogenesis]]
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