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| Line 3: |
Line 3: |
| | <StructureSection load='6cca' size='340' side='right'caption='[[6cca]], [[Resolution|resolution]] 1.75Å' scene=''> | | <StructureSection load='6cca' size='340' side='right'caption='[[6cca]], [[Resolution|resolution]] 1.75Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6cca]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"polyangium_compositum"_thaxter_1904 "polyangium compositum" thaxter 1904]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CCA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CCA FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6cca]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sorangium_cellulosum Sorangium cellulosum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CCA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6CCA FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">dszA, disA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=56 "Polyangium compositum" Thaxter 1904])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cca OCA], [http://pdbe.org/6cca PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cca RCSB], [http://www.ebi.ac.uk/pdbsum/6cca PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cca ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6cca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cca OCA], [https://pdbe.org/6cca PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6cca RCSB], [https://www.ebi.ac.uk/pdbsum/6cca PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6cca ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/Q4U447_SORCE Q4U447_SORCE] |
| - | The methyl substituents in products of trans-acyltransferase assembly lines are usually incorporated by S-adenosyl-methionine (SAM)-dependent methyltransferase (MT) domains. The gem-dimethyl moieties within the polyketide disorazol are installed through the iterative action of an MT in the third module of its assembly line. The 1.75-A-resolution crystal structure of this MT helps elucidate how it catalyzes the addition of two methyl groups. Activity assays of point mutants on beta-ketoacyl chains linked to an acyl carrier protein and N-acetylcysteamine provide additional insights into the roles of active site residues. The replacement of an alanine with a phenylalanine at an apparent gatekeeping position resulted in more monomethylation than dimethylation. MTs may form an interface with ketoreductases (KRs) and even mediate the docking of trans-acyltransferase assembly line polypeptides through this association.
| + | |
| - | | + | |
| - | Structural and Functional Studies of a gem-Dimethylating Methyltransferase from a trans-Acyltransferase Assembly Line.,Meinke JL, Mehaffey MR, Wagner DT, Sun N, Zhang Z, Brodbelt JS, Keatinge-Clay AT ACS Chem Biol. 2018 Nov 9. doi: 10.1021/acschembio.8b00733. PMID:30371052<ref>PMID:30371052</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 6cca" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Polyangium compositum thaxter 1904]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Keatinge-Clay, A T]] | + | [[Category: Sorangium cellulosum]] |
| - | [[Category: Meinke, J L]] | + | [[Category: Keatinge-Clay AT]] |
| - | [[Category: Disorazol]]
| + | [[Category: Meinke JL]] |
| - | [[Category: Gem-dimethyl]]
| + | |
| - | [[Category: Methyltransferase]] | + | |
| - | [[Category: Polyketide assembly line]]
| + | |
| - | [[Category: Transferase]]
| + | |
