6dj4

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==Crystal Structure of Bacillus thuringiensis Cry1A.105 Tryptic Core==
==Crystal Structure of Bacillus thuringiensis Cry1A.105 Tryptic Core==
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<StructureSection load='6dj4' size='340' side='right' caption='[[6dj4]], [[Resolution|resolution]] 3.01&Aring;' scene=''>
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<StructureSection load='6dj4' size='340' side='right'caption='[[6dj4]], [[Resolution|resolution]] 3.01&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6dj4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_cereus_var._thuringiensis"_smith_et_al._1952 "bacillus cereus var. thuringiensis" smith et al. 1952]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DJ4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DJ4 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6dj4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_thuringiensis Bacillus thuringiensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DJ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6DJ4 FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cryIA(c) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1428 "Bacillus cereus var. thuringiensis" Smith et al. 1952])</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.01&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dj4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dj4 OCA], [http://pdbe.org/6dj4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dj4 RCSB], [http://www.ebi.ac.uk/pdbsum/6dj4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dj4 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6dj4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dj4 OCA], [https://pdbe.org/6dj4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6dj4 RCSB], [https://www.ebi.ac.uk/pdbsum/6dj4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6dj4 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/CR1FA_BACTA CR1FA_BACTA] Promotes colloidosmotic lysis by binding to the midgut epithelial cells of many lepidopteran larvae.[https://www.uniprot.org/uniprot/CR1AB_BACTA CR1AB_BACTA] Promotes colloidosmotic lysis by binding to the midgut epithelial cells of many lepidopteran larvae.
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The lepidopteran-active Cry1A.105 protein is a chimeric three-domain insecticidal toxin with distinct structural domains derived from the naturally occurring Cry1Ab, Cry1Ac and Cry1F proteins from the soil bacterium Bacillus thuringiensis (Bt). The X-ray crystal structure of the Cry1A.105 tryptic core at 3.0A resolution demonstrates its high structural similarity to the tryptic core of Cry1Ac. Bioinformatics analyses demonstrate that Cry1A.105 has no significant amino acid sequence similarity to known allergens or mammalian toxins, which is the same conclusion reached for its component domains. Like its intact donor proteins, Cry1A.105 was heat labile at temperatures &gt;/=75 degrees C and degraded upon exposure to gastrointestinal proteases. No adverse effects were observed in mice when Cry1A.105 was dosed orally at 2451mg/kg body weight. Therefore, the weight of evidence supports that Cry1A.105 is safe for human and animal consumption. These results support the conclusion that the safety of a chimeric protein for human or animal consumption can be evaluated in the context of the safety of its donor proteins.
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Safety of the Bacillus thuringiensis-derived Cry1A.105 protein: Evidence that domain exchange preserves mode of action and safety.,Wang C, Li W, Kessenich CR, Petrick JS, Rydel TJ, Sturman EJ, Lee TC, Glenn KC, Edrington TC Regul Toxicol Pharmacol. 2018 Sep 6;99:50-60. doi: 10.1016/j.yrtph.2018.09.003. PMID:30196079<ref>PMID:30196079</ref>
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==See Also==
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*[[Delta-endotoxin|Delta-endotoxin]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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*[[Pesticidal crystal protein|Pesticidal crystal protein]]
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</div>
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<div class="pdbe-citations 6dj4" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Bacillus cereus var. thuringiensis smith et al. 1952]]
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[[Category: Bacillus thuringiensis]]
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[[Category: Lee, T C]]
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[[Category: Large Structures]]
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[[Category: Rydel, T J]]
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[[Category: Lee TC]]
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[[Category: Sturman, E J]]
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[[Category: Rydel TJ]]
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[[Category: Pesticidal crystal protein]]
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[[Category: Sturman EJ]]
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[[Category: Toxin]]
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Current revision

Crystal Structure of Bacillus thuringiensis Cry1A.105 Tryptic Core

PDB ID 6dj4

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