6m9j

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Current revision (14:41, 13 March 2024) (edit) (undo)
 
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<StructureSection load='6m9j' size='340' side='right'caption='[[6m9j]], [[Resolution|resolution]] 0.90&Aring;' scene=''>
<StructureSection load='6m9j' size='340' side='right'caption='[[6m9j]], [[Resolution|resolution]] 0.90&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6m9j]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6M9J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6M9J FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6m9j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_syringae_pv._syringae Pseudomonas syringae pv. syringae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6M9J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6M9J FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6m7m|6m7m]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron crystallography, [[Resolution|Resolution]] 0.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6m9j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m9j OCA], [http://pdbe.org/6m9j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6m9j RCSB], [http://www.ebi.ac.uk/pdbsum/6m9j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6m9j ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6m9j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m9j OCA], [https://pdbe.org/6m9j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6m9j RCSB], [https://www.ebi.ac.uk/pdbsum/6m9j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6m9j ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/ICEN_PSESY ICEN_PSESY]] Ice nucleation proteins enable bacteria to nucleate crystallization in supercooled water.
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[https://www.uniprot.org/uniprot/ICEN_PSESY ICEN_PSESY] Ice nucleation proteins enable bacteria to nucleate crystallization in supercooled water.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The ice-nucleation protein InaZ from Pseudomonas syringae contains a large number of degenerate repeats that span more than a quarter of its sequence and include the segment GSTSTA. Ab initio structures of this repeat segment, resolved to 1.1 A by microfocus X-ray crystallography and to 0.9 A by the cryo-EM method MicroED, were determined from both racemic and homochiral crystals. The benefits of racemic protein crystals for structure determination by MicroED were evaluated and it was confirmed that the phase restriction introduced by crystal centrosymmetry increases the number of successful trials during the ab initio phasing of the electron diffraction data. Both homochiral and racemic GSTSTA form amyloid-like protofibrils with labile, corrugated antiparallel beta-sheets that mate face to back. The racemic GSTSTA protofibril represents a new class of amyloid assembly in which all-left-handed sheets mate with their all-right-handed counterparts. This determination of racemic amyloid assemblies by MicroED reveals complex amyloid architectures and illustrates the racemic advantage in macromolecular crystallography, now with submicrometre-sized crystals.
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Homochiral and racemic MicroED structures of a peptide repeat from the ice-nucleation protein InaZ.,Zee CT, Glynn C, Gallagher-Jones M, Miao J, Santiago CG, Cascio D, Gonen T, Sawaya MR, Rodriguez JA IUCrJ. 2019 Jan 24;6(Pt 2):197-205. doi: 10.1107/S2052252518017621. eCollection, 2019 Mar 1. PMID:30867917<ref>PMID:30867917</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6m9j" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Cascio, D]]
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[[Category: Pseudomonas syringae pv. syringae]]
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[[Category: Gallagher-Jones, M]]
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[[Category: Cascio D]]
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[[Category: Glynn, C]]
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[[Category: Gallagher-Jones M]]
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[[Category: Gonen, T]]
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[[Category: Glynn C]]
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[[Category: Miao, J]]
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[[Category: Gonen T]]
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[[Category: Rodriguez, J A]]
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[[Category: Miao J]]
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[[Category: Santiago, C G]]
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[[Category: Rodriguez JA]]
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[[Category: Sawaya, M R]]
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[[Category: Santiago CG]]
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[[Category: Zee, C]]
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[[Category: Sawaya MR]]
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[[Category: Amyloid]]
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[[Category: Zee C]]
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[[Category: Ice nucleation]]
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[[Category: Inaz]]
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[[Category: Microed]]
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[[Category: Protein fibril]]
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[[Category: Pseudomonas syringae]]
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[[Category: Racemic]]
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Current revision

Racemic-GSTSTA from degenerate octameric repeats in InaZ, residues 707-712

PDB ID 6m9j

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