6mlt

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<StructureSection load='6mlt' size='340' side='right'caption='[[6mlt]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
<StructureSection load='6mlt' size='340' side='right'caption='[[6mlt]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6mlt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibch Vibch]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MLT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MLT FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6mlt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae_O1_biovar_El_Tor_str._N16961 Vibrio cholerae O1 biovar El Tor str. N16961]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MLT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6MLT FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">VC_1888 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243277 VIBCH])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mlt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mlt OCA], [http://pdbe.org/6mlt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mlt RCSB], [http://www.ebi.ac.uk/pdbsum/6mlt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mlt ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6mlt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mlt OCA], [https://pdbe.org/6mlt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6mlt RCSB], [https://www.ebi.ac.uk/pdbsum/6mlt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6mlt ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q9KQW0_VIBCH Q9KQW0_VIBCH]
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Growth of the cholera bacterium Vibrio cholerae in a biofilm community contributes to both its pathogenicity and survival in aquatic environmental niches. The major components of V. cholerae biofilms include Vibrio polysaccharide (VPS) and the extracellular matrix proteins RbmA, RbmC, and Bap1. To further elucidate the previously observed overlapping roles of Bap1 and RbmC in biofilm architecture and surface attachment, here we investigated the structural and functional properties of Bap1. Soluble expression of Bap1 was possible only after the removal of an internal 57-amino-acid-long hydrophobic insertion sequence. The crystal structure of Bap1 at 1.9 A resolution revealed a two-domain assembly made up of an eight-bladed beta-propeller interrupted by a beta-prism domain. The structure also revealed metal-binding sites within canonical calcium blade motifs, which appear to have structural rather than functional roles. Contrary to results previously observed with RbmC, the Bap1 beta-prism domain did not exhibit affinity for complex N-glycans, suggesting an altered role of this domain in biofilm-surface adhesion. Native polyacrylamide gel shift analysis did suggest that Bap1 exhibits lectin activity with a preference for anionic or linear polysaccharides. Our results suggest a model for V. cholerae biofilms in which Bap1 and RbmC play dominant but differing adhesive roles in biofilms, allowing bacterial attachment to diverse environmental or host surfaces.
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The 1.9 A crystal structure of the extracellular matrix protein Bap1 from Vibrio cholerae provides insights into bacterial biofilm adhesion.,Kaus K, Biester A, Chupp E, Lu J, Visudharomn C, Olson R J Biol Chem. 2019 Oct 4;294(40):14499-14511. doi: 10.1074/jbc.RA119.008335. Epub , 2019 Aug 22. PMID:31439670<ref>PMID:31439670</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6mlt" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Vibch]]
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[[Category: Vibrio cholerae O1 biovar El Tor str. N16961]]
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[[Category: Biester, A]]
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[[Category: Biester A]]
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[[Category: Chupp, E]]
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[[Category: Chupp E]]
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[[Category: Kaus, K]]
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[[Category: Kaus K]]
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[[Category: Lu, K]]
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[[Category: Lu K]]
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[[Category: Olson, R]]
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[[Category: Olson R]]
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[[Category: Vidsudharomn, C]]
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[[Category: Vidsudharomn C]]
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[[Category: Biofilm matrix protein]]
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[[Category: Carbohydrate-binding protein]]
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[[Category: Metal-binding protein]]
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[[Category: Secreted protein]]
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[[Category: Sugar binding protein]]
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Current revision

Crystal structure of the V. cholerae biofilm matrix protein Bap1

PDB ID 6mlt

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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