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| | <StructureSection load='6mlx' size='340' side='right'caption='[[6mlx]], [[Resolution|resolution]] 2.00Å' scene=''> | | <StructureSection load='6mlx' size='340' side='right'caption='[[6mlx]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6mlx]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"microspironema_pallidum"_(schaudinn_and_hoffmann_1905)_stiles_and_pfender_1905 "microspironema pallidum" (schaudinn and hoffmann 1905) stiles and pfender 1905]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MLX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MLX FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6mlx]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Treponema_pallidum Treponema pallidum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MLX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6MLX FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mlx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mlx OCA], [http://pdbe.org/6mlx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mlx RCSB], [http://www.ebi.ac.uk/pdbsum/6mlx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mlx ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6mlx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mlx OCA], [https://pdbe.org/6mlx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6mlx RCSB], [https://www.ebi.ac.uk/pdbsum/6mlx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6mlx ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/O06686_TREPL O06686_TREPL] |
| - | The phylogenetically divergent spirochete bacterium Treponema pallidum subsp. pallidum is the causative agent of syphilis. Central to the capacity of T. pallidum to establish infection is the ability of the pathogen to attach to a diversity of host cells. Many pathogenic bacteria employ leucine-rich repeat (LRR) domain-containing proteins to mediate protein-protein interactions, including attachment to host components and establishment of infection. Intriguingly, T. pallidum expresses only one putative LRR domain-containing protein (Tp0225) with an unknown function. In an effort to ascribe a function to Tp0225, a comprehensive phylogenetic analysis was first performed; this investigation revealed that Tp0225 clusters with the pathogenic clade of treponemes. Its crystal structure was then determined to 2.0 A resolution using Pt SAD phasing, which revealed a noncanonical architecture containing a hexameric LRR core with a discontinuous beta-sheet bridged by solvent molecules. Furthermore, a surface-exposed, hydrophobic pocket, which was found in Tp0225 but is largely absent in canonical LRR domains from other pathogenic bacteria, may serve to coordinate a hydrophobic ligand. Overall, this study provides the first structural characterization of the sole LRR domain-containing protein from T. pallidum and offers insight into the unique molecular landscape of this important human pathogen.
| + | |
| - | | + | |
| - | Structural characterization of Treponema pallidum Tp0225 reveals an unexpected leucine-rich repeat architecture.,Ramaswamy R, Houston S, Loveless B, Cameron CE, Boulanger MJ Acta Crystallogr F Struct Biol Commun. 2019 Jul 1;75(Pt 7):489-495. doi:, 10.1107/S2053230X19007726. Epub 2019 Jun 26. PMID:31282868<ref>PMID:31282868</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 6mlx" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Boulanger, M J]] | + | [[Category: Treponema pallidum]] |
| - | [[Category: Cameron, C E]] | + | [[Category: Boulanger MJ]] |
| - | [[Category: Houston, S]] | + | [[Category: Cameron CE]] |
| - | [[Category: Loveless, B C]] | + | [[Category: Houston S]] |
| - | [[Category: Ramaswamy, R]] | + | [[Category: Loveless BC]] |
| - | [[Category: Periplasmic lrr]]
| + | [[Category: Ramaswamy R]] |
| - | [[Category: Secreted]]
| + | |
| - | [[Category: T. pallidum]]
| + | |
| - | [[Category: Unknown function]]
| + | |
