This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


6mvn

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (14:45, 13 March 2024) (edit) (undo)
 
Line 3: Line 3:
<StructureSection load='6mvn' size='340' side='right'caption='[[6mvn]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
<StructureSection load='6mvn' size='340' side='right'caption='[[6mvn]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[6mvn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseab Pseab]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MVN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MVN FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[6mvn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_UCBPP-PA14 Pseudomonas aeruginosa UCBPP-PA14]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MVN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6MVN FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3M5:3-OXO-N-[(3S)-2-OXOTETRAHYDROFURAN-3-YL]DECANAMIDE'>3M5</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
-
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lasR, PA14_45960 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208963 PSEAB])</td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3M5:3-OXO-N-[(3S)-2-OXOTETRAHYDROFURAN-3-YL]DECANAMIDE'>3M5</scene></td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mvn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mvn OCA], [http://pdbe.org/6mvn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mvn RCSB], [http://www.ebi.ac.uk/pdbsum/6mvn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mvn ProSAT]</span></td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6mvn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mvn OCA], [https://pdbe.org/6mvn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6mvn RCSB], [https://www.ebi.ac.uk/pdbsum/6mvn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6mvn ProSAT]</span></td></tr>
</table>
</table>
-
<div style="background-color:#fffaf0;">
+
== Function ==
-
== Publication Abstract from PubMed ==
+
[https://www.uniprot.org/uniprot/A0A0H2Z901_PSEAB A0A0H2Z901_PSEAB]
-
Quorum sensing is a cell-cell communication process that bacteria use to orchestrate group behaviors. Quorum sensing is mediated by signal molecules called autoinducers. Autoinducers are often structurally similar, raising questions concerning how bacteria distinguish among them. Here, we use the Pseudomonas aeruginosa LasR quorum-sensing receptor to explore signal discrimination. The cognate autoinducer, 3OC12 homoserine lactone (3OC12HSL), is a more potent activator of LasR than other homoserine lactones. However, other homoserine lactones can elicit LasR-dependent quorum-sensing responses, showing that LasR displays ligand promiscuity. We identify mutants that alter which homoserine lactones LasR detects. Substitution at residue S129 decreases the LasR response to 3OC12HSL, while enhancing discrimination against noncognate autoinducers. Conversely, the LasR L130F mutation increases the potency of 3OC12HSL and other homoserine lactones. We solve crystal structures of LasR ligand-binding domains complexed with noncognate autoinducers. Comparison with existing structures reveals that ligand selectivity/sensitivity is mediated by a flexible loop near the ligand-binding site. We show that LasR variants with modified ligand preferences exhibit altered quorum-sensing responses to autoinducers in vivo. We suggest that possessing some ligand promiscuity endows LasR with the ability to optimally regulate quorum-sensing traits.
+
-
Structural determinants driving homoserine lactone ligand selection in the Pseudomonas aeruginosa LasR quorum-sensing receptor.,McCready AR, Paczkowski JE, Henke BR, Bassler BL Proc Natl Acad Sci U S A. 2019 Jan 2;116(1):245-254. doi:, 10.1073/pnas.1817239116. Epub 2018 Dec 17. PMID:30559209<ref>PMID:30559209</ref>
+
==See Also==
-
 
+
*[[Transcriptional activator 3D structures|Transcriptional activator 3D structures]]
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 6mvn" style="background-color:#fffaf0;"></div>
+
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
-
[[Category: Pseab]]
+
[[Category: Pseudomonas aeruginosa UCBPP-PA14]]
-
[[Category: Bassler, B L]]
+
[[Category: Bassler BL]]
-
[[Category: Paczkowski, J E]]
+
[[Category: Paczkowski JE]]
-
[[Category: Transcription]]
+
-
[[Category: Transcriptional activator]]
+

Current revision

LasR LBD L130F:3OC10HSL complex

PDB ID 6mvn

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6mvn"
Personal tools