6n7o

<table><tr><td colspan='2'>[[6n7o]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_phage_pgil01 Bacillus phage pgil01]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N7O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6N7O FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6n7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n7o OCA], [http://pdbe.org/6n7o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6n7o RCSB], [http://www.ebi.ac.uk/pdbsum/6n7o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6n7o ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

Bacteria identify and respond to DNA damage using the SOS response. LexA, a central repressor in the response, has been implicated in the regulation of lysogeny in various temperate bacteriophages. During infection of Bacillus thuringiensis with GIL01 bacteriophage, LexA represses the SOS response and the phage lytic cycle by binding DNA, an interaction further stabilized upon binding of a viral protein, gp7. Here we report the crystallographic structure of phage-borne gp7 at 1.7-A resolution, and characterize the 4:2 stoichiometry and potential interaction with LexA using surface plasmon resonance, static light scattering, and small-angle X-ray scattering. These data suggest that gp7 stabilizes LexA binding to operator DNA via coordination of the N- and C-terminal domains of LexA. Furthermore, we have found that gp7 can interact with LexA from Staphylococcus aureus, a significant human pathogen. Our results provide structural evidence as to how phage factors can directly associate with LexA to modulate the SOS response.

 

Structural Insights into Bacteriophage GIL01 gp7 Inhibition of Host LexA Repressor.,Caveney NA, Pavlin A, Caballero G, Bahun M, Hodnik V, de Castro L, Fornelos N, Butala M, Strynadka NCJ Structure. 2019 Apr 10. pii: S0969-2126(19)30115-7. doi:, 10.1016/j.str.2019.03.019. PMID:31056420<ref>PMID:31056420</ref>

 

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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<references/>

[[Category: Bacillus phage pgil01]]

[[Category: Caveney, N A]]

[[Category: Strynadka, N C.J]]

[[Category: Viral protein]]