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| | <SX load='6ny1' size='340' side='right' viewer='molstar' caption='[[6ny1]], [[Resolution|resolution]] 4.20Å' scene=''> | | <SX load='6ny1' size='340' side='right' viewer='molstar' caption='[[6ny1]], [[Resolution|resolution]] 4.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6ny1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Deltaproteobacteria_bacterium Deltaproteobacteria bacterium]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=6e7a 6e7a]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NY1 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6NY1 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6ny1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Deltaproteobacteria_bacterium Deltaproteobacteria bacterium]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=6e7a 6e7a]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NY1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NY1 FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.2Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">DD725_10130 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2026735 Deltaproteobacteria bacterium])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ny1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ny1 OCA], [https://pdbe.org/6ny1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ny1 RCSB], [https://www.ebi.ac.uk/pdbsum/6ny1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ny1 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6ny1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ny1 OCA], [http://pdbe.org/6ny1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ny1 RCSB], [http://www.ebi.ac.uk/pdbsum/6ny1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ny1 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/A0A357BT59_9DELT A0A357BT59_9DELT] |
| - | The RNA-guided CRISPR-associated (Cas) proteins Cas9 and Cas12a provide adaptive immunity against invading nucleic acids, and function as powerful tools for genome editing in a wide range of organisms. Here we reveal the underlying mechanisms of a third, fundamentally distinct RNA-guided genome-editing platform named CRISPR-CasX, which uses unique structures for programmable double-stranded DNA binding and cleavage. Biochemical and in vivo data demonstrate that CasX is active for Escherichia coli and human genome modification. Eight cryo-electron microscopy structures of CasX in different states of assembly with its guide RNA and double-stranded DNA substrates reveal an extensive RNA scaffold and a domain required for DNA unwinding. These data demonstrate how CasX activity arose through convergent evolution to establish an enzyme family that is functionally separate from both Cas9 and Cas12a.
| + | |
| - | | + | |
| - | CasX enzymes comprise a distinct family of RNA-guided genome editors.,Liu JJ, Orlova N, Oakes BL, Ma E, Spinner HB, Baney KLM, Chuck J, Tan D, Knott GJ, Harrington LB, Al-Shayeb B, Wagner A, Brotzmann J, Staahl BT, Taylor KL, Desmarais J, Nogales E, Doudna JA Nature. 2019 Feb;566(7743):218-223. doi: 10.1038/s41586-019-0908-x. Epub 2019 Feb, 4. PMID:30718774<ref>PMID:30718774</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 6ny1" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </SX> | | </SX> |
| | [[Category: Deltaproteobacteria bacterium]] | | [[Category: Deltaproteobacteria bacterium]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Doudna, J A]] | + | [[Category: Doudna JA]] |
| - | [[Category: Liu, J J]] | + | [[Category: Liu JJ]] |
| - | [[Category: Nogales, E]] | + | [[Category: Nogales E]] |
| - | [[Category: Orlova, N]] | + | [[Category: Orlova N]] |
| - | [[Category: Casx]]
| + | |
| - | [[Category: Crispr]]
| + | |
| - | [[Category: Rna binding protein-rna-dna complex]]
| + | |
| - | [[Category: Sgrna]]
| + | |
| - | [[Category: Target dna]]
| + | |
