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6ny1

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Current revision (14:49, 13 March 2024) (edit) (undo)
 
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<SX load='6ny1' size='340' side='right' viewer='molstar' caption='[[6ny1]], [[Resolution|resolution]] 4.20&Aring;' scene=''>
<SX load='6ny1' size='340' side='right' viewer='molstar' caption='[[6ny1]], [[Resolution|resolution]] 4.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6ny1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Deltaproteobacteria_bacterium Deltaproteobacteria bacterium]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=6e7a 6e7a]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NY1 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6NY1 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6ny1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Deltaproteobacteria_bacterium Deltaproteobacteria bacterium]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=6e7a 6e7a]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NY1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NY1 FirstGlance]. <br>
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</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.2&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">DD725_10130 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2026735 Deltaproteobacteria bacterium])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ny1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ny1 OCA], [https://pdbe.org/6ny1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ny1 RCSB], [https://www.ebi.ac.uk/pdbsum/6ny1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ny1 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6ny1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ny1 OCA], [http://pdbe.org/6ny1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ny1 RCSB], [http://www.ebi.ac.uk/pdbsum/6ny1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ny1 ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/A0A357BT59_9DELT A0A357BT59_9DELT]
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The RNA-guided CRISPR-associated (Cas) proteins Cas9 and Cas12a provide adaptive immunity against invading nucleic acids, and function as powerful tools for genome editing in a wide range of organisms. Here we reveal the underlying mechanisms of a third, fundamentally distinct RNA-guided genome-editing platform named CRISPR-CasX, which uses unique structures for programmable double-stranded DNA binding and cleavage. Biochemical and in vivo data demonstrate that CasX is active for Escherichia coli and human genome modification. Eight cryo-electron microscopy structures of CasX in different states of assembly with its guide RNA and double-stranded DNA substrates reveal an extensive RNA scaffold and a domain required for DNA unwinding. These data demonstrate how CasX activity arose through convergent evolution to establish an enzyme family that is functionally separate from both Cas9 and Cas12a.
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CasX enzymes comprise a distinct family of RNA-guided genome editors.,Liu JJ, Orlova N, Oakes BL, Ma E, Spinner HB, Baney KLM, Chuck J, Tan D, Knott GJ, Harrington LB, Al-Shayeb B, Wagner A, Brotzmann J, Staahl BT, Taylor KL, Desmarais J, Nogales E, Doudna JA Nature. 2019 Feb;566(7743):218-223. doi: 10.1038/s41586-019-0908-x. Epub 2019 Feb, 4. PMID:30718774<ref>PMID:30718774</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6ny1" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</SX>
</SX>
[[Category: Deltaproteobacteria bacterium]]
[[Category: Deltaproteobacteria bacterium]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Doudna, J A]]
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[[Category: Doudna JA]]
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[[Category: Liu, J J]]
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[[Category: Liu JJ]]
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[[Category: Nogales, E]]
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[[Category: Nogales E]]
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[[Category: Orlova, N]]
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[[Category: Orlova N]]
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[[Category: Casx]]
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[[Category: Crispr]]
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[[Category: Rna binding protein-rna-dna complex]]
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[[Category: Sgrna]]
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[[Category: Target dna]]
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Current revision

CasX-gRNA-DNA(30bp) State II

6ny1, resolution 4.20Å

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OCA

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