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| | <StructureSection load='6o59' size='340' side='right'caption='[[6o59]], [[Resolution|resolution]] 2.79Å' scene=''> | | <StructureSection load='6o59' size='340' side='right'caption='[[6o59]], [[Resolution|resolution]] 2.79Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6o59]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_14581 Atcc 14581]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O59 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6O59 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6o59]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Priestia_megaterium Priestia megaterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O59 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6O59 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gerAA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1404 ATCC 14581])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.79Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6o59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6o59 OCA], [http://pdbe.org/6o59 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6o59 RCSB], [http://www.ebi.ac.uk/pdbsum/6o59 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6o59 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6o59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6o59 OCA], [https://pdbe.org/6o59 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6o59 RCSB], [https://www.ebi.ac.uk/pdbsum/6o59 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6o59 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/O07504_PRIMG O07504_PRIMG] |
| - | Germination of Bacillus spores is induced by the interaction of specific nutrient molecules with germinant receptors (GRs) localized in the spore's inner membrane. GRs typically consist of three subunits referred to as A, B, and C, although functions of individual subunits are not known. Here we present the crystal structure of the N-terminal domain (NTD) of the A subunit of the Bacillus megaterium GerK3 GR, revealing two distinct globular subdomains bisected by a cleft, a fold with strong homology to substrate-binding proteins in bacterial ABC transporters. Molecular docking, chemical shift perturbation measurement, and mutagenesis coupled with spore germination analyses support a proposed model that the interface between the two subdomains in the NTD of GR A subunits serves as the germinant binding site and plays a critical role in spore germination. Our findings provide a conceptual framework for understanding the germinant recruitment mechanism by which GRs trigger spore germination.
| + | |
| - | | + | |
| - | Structural and functional analyses of the N-terminal domain of the A subunit of a Bacillus megaterium spore germinant receptor.,Li Y, Jin K, Perez-Valdespino A, Federkiewicz K, Davis A, Maciejewski MW, Setlow P, Hao B Proc Natl Acad Sci U S A. 2019 May 21. pii: 1903675116. doi:, 10.1073/pnas.1903675116. PMID:31113879<ref>PMID:31113879</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 6o59" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 14581]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Hao, B]] | + | [[Category: Priestia megaterium]] |
| - | [[Category: Li, Y]] | + | [[Category: Hao B]] |
| - | [[Category: Bacillus]] | + | [[Category: Li Y]] |
| - | [[Category: Spore]]
| + | |
| - | [[Category: Spore germinant recepter]]
| + | |
| - | [[Category: Spore germination]]
| + | |
| - | [[Category: Transport protein]]
| + | |
