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| | <StructureSection load='6oh4' size='340' side='right'caption='[[6oh4]], [[Resolution|resolution]] 3.38Å' scene=''> | | <StructureSection load='6oh4' size='340' side='right'caption='[[6oh4]], [[Resolution|resolution]] 3.38Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6oh4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OH4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6OH4 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6oh4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OH4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6OH4 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C5P:CYTIDINE-5-MONOPHOSPHATE'>C5P</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.38Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Slc35a1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C5P:CYTIDINE-5-MONOPHOSPHATE'>C5P</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6oh4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6oh4 OCA], [http://pdbe.org/6oh4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6oh4 RCSB], [http://www.ebi.ac.uk/pdbsum/6oh4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6oh4 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6oh4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6oh4 OCA], [https://pdbe.org/6oh4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6oh4 RCSB], [https://www.ebi.ac.uk/pdbsum/6oh4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6oh4 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/S35A1_MOUSE S35A1_MOUSE]] Transport CMP-sialic acid from the cytosol into Golgi vesicles where glycosyltransferases function. | + | [https://www.uniprot.org/uniprot/S35A1_MOUSE S35A1_MOUSE] Transport CMP-sialic acid from the cytosol into Golgi vesicles where glycosyltransferases function. |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | Nucleotide-sugar transporters (NSTs) are critical components of the cellular glycosylation machinery. They transport nucleotide-sugar conjugates into the Golgi lumen, where they are used for the glycosylation of proteins and lipids, and they then subsequently transport the nucleotide monophosphate byproduct back to the cytoplasm. Dysregulation of human NSTs causes several debilitating diseases, and NSTs are virulence factors for many pathogens. Here we present the first crystal structures of a mammalian NST, the mouse CMP-sialic acid transporter (mCST), in complex with its physiological substrates CMP and CMP-sialic acid. Detailed visualization of extensive protein-substrate interactions explains the mechanisms governing substrate selectivity. Further structural analysis of mCST's unique lumen-facing partially-occluded conformation, coupled with the characterization of substrate-induced quenching of mCST's intrinsic tryptophan fluorescence, reveals the concerted conformational transitions that occur during substrate transport. These results provide a framework for understanding the effects of disease-causing mutations and the mechanisms of this diverse family of transporters.
| + | |
| - | | + | |
| - | Structural basis for mammalian nucleotide sugar transport.,Ahuja S, Whorton MR Elife. 2019 Apr 15;8. pii: 45221. doi: 10.7554/eLife.45221. PMID:30985278<ref>PMID:30985278</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 6oh4" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Mus musculus]] |
| - | [[Category: Ahuja, S]] | + | [[Category: Ahuja S]] |
| - | [[Category: Whorton, M R]] | + | [[Category: Whorton MR]] |
| - | [[Category: Drug/metabolite transporter]]
| + | |
| - | [[Category: Glycosylation]]
| + | |
| - | [[Category: Nucleotide-sugar]]
| + | |
| - | [[Category: Sialic acid]]
| + | |
| - | [[Category: Transport protein]]
| + | |
