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| | <StructureSection load='6pbz' size='340' side='right'caption='[[6pbz]], [[Resolution|resolution]] 2.48Å' scene=''> | | <StructureSection load='6pbz' size='340' side='right'caption='[[6pbz]], [[Resolution|resolution]] 2.48Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6pbz]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PBZ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6PBZ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6pbz]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PBZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6PBZ FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.475Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gppA, gpp, b3779, JW5603 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Guanosine-5'-triphosphate,3'-diphosphate_diphosphatase Guanosine-5'-triphosphate,3'-diphosphate diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.40 3.6.1.40] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6pbz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pbz OCA], [https://pdbe.org/6pbz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6pbz RCSB], [https://www.ebi.ac.uk/pdbsum/6pbz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6pbz ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6pbz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pbz OCA], [http://pdbe.org/6pbz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6pbz RCSB], [http://www.ebi.ac.uk/pdbsum/6pbz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6pbz ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/GPPA_ECOLI GPPA_ECOLI]] Catalyzes the conversion of pppGpp to ppGpp. Guanosine pentaphosphate (pppGpp) is a cytoplasmic signaling molecule which together with ppGpp controls the 'stringent response', an adaptive process that allows bacteria to respond to amino acid starvation, resulting in the coordinated regulation of numerous cellular activities (PubMed:6130093, PubMed:8394006). In vitro, can hydrolyze pppGp (PubMed:6130093). Also has exopolyphosphatase activity, catalyzing the release of orthophosphate by processive hydrolysis of the phosphoanyhydride bonds of polyphosphate chains (1000 residues) (PubMed:8394006).<ref>PMID:6130093</ref> <ref>PMID:8394006</ref> | + | [https://www.uniprot.org/uniprot/GPPA_ECOLI GPPA_ECOLI] Catalyzes the conversion of pppGpp to ppGpp. Guanosine pentaphosphate (pppGpp) is a cytoplasmic signaling molecule which together with ppGpp controls the 'stringent response', an adaptive process that allows bacteria to respond to amino acid starvation, resulting in the coordinated regulation of numerous cellular activities (PubMed:6130093, PubMed:8394006). In vitro, can hydrolyze pppGp (PubMed:6130093). Also has exopolyphosphatase activity, catalyzing the release of orthophosphate by processive hydrolysis of the phosphoanyhydride bonds of polyphosphate chains (1000 residues) (PubMed:8394006).<ref>PMID:6130093</ref> <ref>PMID:8394006</ref> |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | Rapid adaptation to environmental changes is crucial for bacterial survival. Almost all bacteria possess a conserved stringent response system to prompt transcriptional and metabolic responses toward stress. The adaptive process relies on alarmones, guanosine pentaphosphate (pppGpp) and tetraphosphate (ppGpp), to regulate global gene expression. The ppGpp is more potent than pppGpp in the regulatory activity and pppGpp phosphohydrolase (GppA) plays a key role in (p)ppGpp homeostasis. Sharing a similar domain structure, GppA is indistinguishable from exopolyphosphatase (PPX), which mediates the metabolism of cellular inorganic polyphosphate. Here, our phylogenetic analysis of PPX/GppA homologs in bacteria shows a wide distribution with several distinct subfamilies, and our structural and functional analysis of Escherichia coli GppA and Helicobacter pylori PPX/GppA reveals unique properties of each homolog. These results explain how each homolog possesses its distinct functionality.
| + | |
| - | | + | |
| - | Structure and Activity of PPX/GppA Homologs from Escherichia coli and Helicobacter pylori.,Song H, Dharmasena MN, Wang C, Shaw GX, Cherry S, Tropea JE, Jin DJ, Ji X FEBS J. 2019 Nov 2. doi: 10.1111/febs.15120. PMID:31679177<ref>PMID:31679177</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 6pbz" style="background-color:#fffaf0;"></div>
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| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Ecoli]] | + | [[Category: Escherichia coli K-12]] |
| - | [[Category: Guanosine-5'-triphosphate,3'-diphosphate diphosphatase]]
| + | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Ji, X]] | + | [[Category: Ji X]] |
| - | [[Category: Shaw, G X]] | + | [[Category: Shaw GX]] |
| - | [[Category: Song, H]] | + | [[Category: Song H]] |
| - | [[Category: Wang, C]] | + | [[Category: Wang C]] |
| - | [[Category: Gppa]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Ppgpp]]
| + | |
| - | [[Category: Pppgpp]]
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| - | [[Category: Ppx]]
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