6pmo

<table><tr><td colspan='2'>[[6pmo]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PMO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6PMO FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IR:IRIDIUM+ION'>IR</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>

<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6pom|6pom]]</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6pmo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pmo OCA], [http://pdbe.org/6pmo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6pmo RCSB], [http://www.ebi.ac.uk/pdbsum/6pmo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6pmo ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

Amino acid availability in Gram-positive bacteria is monitored by T-box riboswitches. T-boxes directly bind tRNAs, assess their aminoacylation state, and regulate the transcription or translation of downstream genes to maintain nutritional homeostasis. Here, we report cocrystal and cryo-EM structures of Geobacillus kaustophilus and Bacillus subtilis T-box-tRNA complexes, detailing their multivalent, exquisitely selective interactions. The T-box forms a U-shaped molecular vise that clamps the tRNA, captures its 3' end using an elaborate 'discriminator' structure, and interrogates its aminoacylation state using a steric filter fashioned from a wobble base pair. In the absence of aminoacylation, T-boxes clutch tRNAs and form a continuously stacked central spine, permitting transcriptional readthrough or translation initiation. A modeled aminoacyl disrupts tRNA-T-box stacking, severing the central spine and blocking gene expression. Our data establish a universal mechanism of amino acid sensing on tRNAs and gene regulation by T-box riboswitches and exemplify how higher-order RNA-RNA interactions achieve multivalency and specificity.

Structural basis of amino acid surveillance by higher-order tRNA-mRNA interactions.,Li S, Su Z, Lehmann J, Stamatopoulou V, Giarimoglou N, Henderson FE, Fan L, Pintilie GD, Zhang K, Chen M, Ludtke SJ, Wang YX, Stathopoulos C, Chiu W, Zhang J Nat Struct Mol Biol. 2019 Nov 18. pii: 10.1038/s41594-019-0326-7. doi:, 10.1038/s41594-019-0326-7. PMID:31740854<ref>PMID:31740854</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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<div class="pdbe-citations 6pmo" style="background-color:#fffaf0;"></div>

== References ==

<references/>

[[Category: Li, S]]

[[Category: Zhang, J]]

[[Category: Trna]]