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6uec

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Current revision (14:57, 13 March 2024) (edit) (undo)
 
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<StructureSection load='6uec' size='340' side='right'caption='[[6uec]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
<StructureSection load='6uec' size='340' side='right'caption='[[6uec]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6uec]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UEC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6UEC FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6uec]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UEC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6UEC FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=Q5M:4-(naphthalen-1-yl)-4-oxobutanoic+acid'>Q5M</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6uec FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uec OCA], [http://pdbe.org/6uec PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6uec RCSB], [http://www.ebi.ac.uk/pdbsum/6uec PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6uec ProSAT]</span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=Q5M:4-(naphthalen-1-yl)-4-oxobutanoic+acid'>Q5M</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6uec FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uec OCA], [https://pdbe.org/6uec PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6uec RCSB], [https://www.ebi.ac.uk/pdbsum/6uec PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6uec ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/LPXD_PSEAE LPXD_PSEAE]] Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.[HAMAP-Rule:MF_00523]
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[https://www.uniprot.org/uniprot/LPXD_PSEAE LPXD_PSEAE] Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.[HAMAP-Rule:MF_00523]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The lipid A biosynthesis pathway is essential in Pseudomonas aeruginosa. LpxA and LpxD are the first and third enzymes in this pathway respectively, and are regarded as promising antibiotic targets. The unique structural similarities between these two enzymes make them suitable targets for dual-binding inhibitors, a characteristic that would decrease the likelihood of mutational resistance and increase cell-based activity. We report the discovery of multiple small molecule ligands that bind to P. aeruginosa LpxA and LpxD, including dual-binding ligands. Binding poses were determined for select compounds by X-ray crystallography. The new structures reveal a previously uncharacterized magnesium ion residing at the core of the LpxD trimer. In addition, ligand binding in the LpxD active site resulted in conformational changes in the distal C-terminal helix-bundle, which forms extensive contacts with acyl carrier protein (ACP) during catalysis. These ligand-dependent conformational changes suggest a potential allosteric influence of reaction intermediates on ACP binding, and vice versa. Taken together, the novel small molecule ligands and their crystal structures provide new chemical scaffolds for ligand discovery targeting lipid A biosynthesis, while revealing structural features of interest for future investigation of LpxD function.
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Discovery of dual-activity small-molecule ligands of Pseudomonas aeruginosa LpxA and LpxD using SPR and X-ray crystallography.,Kroeck KG, Sacco MD, Smith EW, Zhang X, Shoun D, Akhtar A, Darch SE, Cohen F, Andrews LD, Knox JE, Chen Y Sci Rep. 2019 Oct 29;9(1):15450. doi: 10.1038/s41598-019-51844-z. PMID:31664082<ref>PMID:31664082</ref>
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==See Also==
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*[[UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase|UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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*[[UDP-N-acetylglucosamine acyltransferase 3D structures|UDP-N-acetylglucosamine acyltransferase 3D structures]]
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</div>
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<div class="pdbe-citations 6uec" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Chen, Y]]
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[[Category: Pseudomonas aeruginosa PAO1]]
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[[Category: Kroeck, K]]
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[[Category: Chen Y]]
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[[Category: Sacco, M]]
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[[Category: Kroeck K]]
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[[Category: Acyl transferase]]
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[[Category: Sacco M]]
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[[Category: Ligand complex]]
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[[Category: Lpxd]]
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[[Category: Transferase]]
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[[Category: Trimer]]
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Current revision

Pseudomonas aeruginosa LpxD Complex Structure with Ligand

PDB ID 6uec

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