6ufj

<table><tr><td colspan='2'>[[6ufj]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UFJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6UFJ FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>

<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=23G:GUANOSINE-5-PHOSPHATE-2,3-CYCLIC+PHOSPHATE'>23G</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ufj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ufj OCA], [http://pdbe.org/6ufj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ufj RCSB], [http://www.ebi.ac.uk/pdbsum/6ufj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ufj ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

Pistol ribozymes constitute a new class of small self-cleaving RNAs. We have solved crystal structures that provide three-dimensional snapshots along the reaction coordinate of pistol phosphodiester cleavage, corresponding to the pre-catalytic state, a vanadate mimic of the transition state, and the product. The resulting architectonic framework dictates the proposed underlying chemical mechanism, which is corroborated by functional assays that involve atomic mutagenesis of the ribozyme. Importantly, a hydrated Mg2+ ion remains innersphere-coordinated to N7-G33 in all three states. This is consistent with its likely role as acid in general acid base catalysis (delta and ss catalysis), supported further by the observation of a more than 1000-fold rate reduction if its positioning is impaired by deletion of the N7 site. Strikingly, the new structures shed light on a second hydrated Mg2+ ion that approaches the scissile phosphate from its binding site in the pre-cleavage state to reach out for water-mediated H-bonding in the cyclophosphate product. Because this second hydrated Mg2+ ion is also close to the 6-oxygen atom of G40 (general base, gamma catalysis), its potential impact on activation of G40 has been experimentally evaluated, however, the major role of the second Mg2+ ion appears to be the stabilization of product conformation. Together, our study delivers a mechanistic understanding of ribozyme-catalyzed backbone cleavage with unprecedented precision.

 

== References ==

[[Category: Falschlunger, C]]

[[Category: Krasheninina, O]]

[[Category: Micura, R]]

[[Category: Patel, D J]]

[[Category: Teplova, M]]

[[Category: Transesterification reaction]]