1t91

From Proteopedia

---

Revision as of 17:15, 12 November 2007

proteopedia linkproteopedia link

spinqualitylabelsall models 1t91, resolution 1.9Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

crystal structure of human small GTPase Rab7(GTP)

Contents 1 Overview 2 Disease 3 About this Structure 4 Reference

Overview

Rab7 regulates vesicle traffic from early to late endosomes, and from late, endosomes to lysosomes. The crystal structure of Rab7-GTP in complex with, the Rab7 binding domain of RILP reveals that Rab7 interacts with RILP, specifically via two distinct areas, with the first one involving the, switch and interswitch regions and the second one consisting of RabSF1 and, RabSF4. Disruption of these interactions by mutations abrogates late, endosomal/lysosomal targeting of Rab7 and RILP. The Rab7 binding domain of, RILP forms a coiled-coil homodimer with two symmetric surfaces to interact, with two separate Rab7-GTP molecules, forming a dyad configuration of, Rab7-RILP(2)-Rab7. Mutations that disrupt RILP dimerization also abolish, its interactions with Rab7-GTP and late endosomal/lysosomal targeting, suggesting that the dimeric form of RILP is a functional unit. Structural, comparison suggests that the combined use of RabSF1 and RabSF4 with the, switch regions may be a general mode of action for most Rab proteins in, regulating membrane trafficking.

Disease

Known disease associated with this structure: Charcot-Marie-Tooth disease, type 2B OMIM:[602298]

About this Structure

1T91 is a Single protein structure of sequence from Homo sapiens with MG and GTP as ligands. Full crystallographic information is available from OCA.

Reference

Structural basis for recruitment of RILP by small GTPase Rab7., Wu M, Wang T, Loh E, Hong W, Song H, EMBO J. 2005 Apr 20;24(8):1491-501. Epub 2005 Mar 31. PMID:15933719

Page seeded by OCA on Mon Nov 12 19:22:16 2007