1r0n
From Proteopedia
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| - | + | {{STRUCTURE_1r0n| PDB=1r0n | SCENE= }} | |
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'''Crystal Structure of Heterodimeric Ecdsyone receptor DNA binding complex''' | '''Crystal Structure of Heterodimeric Ecdsyone receptor DNA binding complex''' | ||
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[[Category: Ozyhar, A.]] | [[Category: Ozyhar, A.]] | ||
[[Category: Rastinejad, F.]] | [[Category: Rastinejad, F.]] | ||
| - | [[Category: | + | [[Category: Dna binding domain]] |
| - | [[Category: | + | [[Category: Ecdysone receptor]] |
| - | [[Category: | + | [[Category: Nuclear receptor]] |
| - | [[Category: | + | [[Category: Rxr]] |
| - | [[Category: | + | [[Category: Ultraspiracle]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:55:47 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 03:55, 3 May 2008
Crystal Structure of Heterodimeric Ecdsyone receptor DNA binding complex
Overview
Ecdysteroids initiate molting and metamorphosis in insects via a heterodimeric receptor consisting of the ecdysone receptor (EcR) and ultraspiracle (USP). The EcR-USP heterodimer preferentially mediates transcription through highly degenerate pseudo-palindromic response elements, resembling inverted repeats of 5'-AGGTCA-3' separated by 1 bp (IR-1). The requirement for a heterodimeric arrangement of EcR-USP subunits to bind to a symmetric DNA is unusual within the nuclear receptor superfamily. We describe the 2.24 A structure of the EcR-USP DNA-binding domain (DBD) heterodimer bound to an idealized IR-1 element. EcR and USP use similar surfaces, and rely on the deformed minor groove of the DNA to establish protein-protein contacts. As retinoid X receptor (RXR) is the mammalian homolog of USP, we also solved the 2.60 A crystal structure of the EcR-RXR DBD heterodimer on IR-1 and found the dimerization and DNA-binding interfaces to be the same as in the EcR-USP complex. Sequence alignments indicate that the EcR-RXR heterodimer is an important model for understanding how the FXR-RXR heterodimer binds to IR-1 sites.
About this Structure
1R0N is a Protein complex structure of sequences from Drosophila melanogaster and Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure of the heterodimeric ecdysone receptor DNA-binding complex., Devarakonda S, Harp JM, Kim Y, Ozyhar A, Rastinejad F, EMBO J. 2003 Nov 3;22(21):5827-40. PMID:14592980 Page seeded by OCA on Sat May 3 06:55:47 2008
