1d8v
From Proteopedia
(Difference between revisions)
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==THE RESTRAINED AND MINIMIZED AVERAGE NMR STRUCTURE OF MAP30.== | ==THE RESTRAINED AND MINIMIZED AVERAGE NMR STRUCTURE OF MAP30.== | ||
| - | <StructureSection load='1d8v' size='340' side='right'caption='[[1d8v | + | <StructureSection load='1d8v' size='340' side='right'caption='[[1d8v]]' scene=''> |
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1d8v]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Momordica_charantia Momordica charantia]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D8V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D8V FirstGlance]. <br> | <table><tr><td colspan='2'>[[1d8v]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Momordica_charantia Momordica charantia]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D8V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D8V FirstGlance]. <br> | ||
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d8v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d8v OCA], [https://pdbe.org/1d8v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d8v RCSB], [https://www.ebi.ac.uk/pdbsum/1d8v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d8v ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d8v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d8v OCA], [https://pdbe.org/1d8v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d8v RCSB], [https://www.ebi.ac.uk/pdbsum/1d8v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d8v ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
| - | + | [https://www.uniprot.org/uniprot/RIP3_MOMCH RIP3_MOMCH] Irreversibly relaxes supercoiled DNA and catalyzes double-stranded breakage. Acts also as a ribosome inactivating protein. | |
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1d8v ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1d8v ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | We present the solution structure of MAP30, a plant protein with anti-HIV and anti-tumor activities. Structural analysis and subsequent biochemical assays lead to several novel discoveries. First, MAP30 acts like a DNA glycosylase/apurinic (ap) lyase, an additional activity distinct from its known RNA N-glycosidase activity toward the 28S rRNA. Glycosylase/ap lyase activity explains MAP30's apparent inhibition of the HIV-1 integrase, MAP30's ability to irreversibly relax supercoiled DNA, and may be an alternative cytotoxic pathway that contributes to MAP30's anti-HIV/anti-tumor activities. Second, two distinct, but contiguous, subsites are responsible for MAP30's glycosylase/ap lyase activity. Third, Mn2+ and Zn2+ interact with negatively charged surfaces next to the catalytic sites, facilitating DNA substrate binding instead of directly participating in catalysis. | ||
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| - | Solution structure of anti-HIV-1 and anti-tumor protein MAP30: structural insights into its multiple functions.,Wang YX, Neamati N, Jacob J, Palmer I, Stahl SJ, Kaufman JD, Huang PL, Huang PL, Winslow HE, Pommier Y, Wingfield PT, Lee-Huang S, Bax A, Torchia DA Cell. 1999 Nov 12;99(4):433-42. PMID:10571185<ref>PMID:10571185</ref> | ||
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 1d8v" style="background-color:#fffaf0;"></div> | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Momordica charantia]] | [[Category: Momordica charantia]] | ||
| - | [[Category: Jacob | + | [[Category: Jacob J]] |
| - | [[Category: Neamati | + | [[Category: Neamati N]] |
| - | [[Category: Palmer | + | [[Category: Palmer I]] |
| - | [[Category: Stahl | + | [[Category: Stahl SJ]] |
| - | [[Category: Wang | + | [[Category: Wang Y-X]] |
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Revision as of 15:47, 13 March 2024
THE RESTRAINED AND MINIMIZED AVERAGE NMR STRUCTURE OF MAP30.
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