8hi1
From Proteopedia
(Difference between revisions)
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8hi1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8hi1 OCA], [https://pdbe.org/8hi1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8hi1 RCSB], [https://www.ebi.ac.uk/pdbsum/8hi1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8hi1 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8hi1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8hi1 OCA], [https://pdbe.org/8hi1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8hi1 RCSB], [https://www.ebi.ac.uk/pdbsum/8hi1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8hi1 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | In the spacer acquisition stage of CRISPR-Cas immunity, spacer orientation and protospacer adjacent motif (PAM) removal are two prerequisites for functional spacer integration. Cas4 has been implicated in both processing the prespacer and determining the spacer orientation. In Cas4-lacking systems, host 3'-5' DnaQ family exonucleases were recently reported to play a Cas4-like role. However, the molecular details of DnaQ functions remain elusive. Here, we characterized the spacer acquisition of the adaptation module of the Streptococcus thermophilus type I-E system, in which a DnaQ domain naturally fuses with Cas2. We presented X-ray crystal structures and cryo-electron microscopy structures of this adaptation module. Our biochemical data showed that DnaQ trimmed PAM-containing and PAM-deficient overhangs with different efficiencies. Based on these results, we proposed a time-dependent model for DnaQ-mediated spacer acquisition to elucidate PAM removal and spacer orientation determination in Cas4-lacking CRISPR-Cas systems. | ||
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| - | DnaQ mediates directional spacer acquisition in the CRISPR-Cas system by a time-dependent mechanism.,Tang D, Jia T, Luo Y, Mou B, Cheng J, Qi S, Yao S, Su Z, Yu Y, Chen Q Innovation (Camb). 2023 Aug 9;4(5):100495. doi: 10.1016/j.xinn.2023.100495. , eCollection 2023 Sep 11. PMID:37663930<ref>PMID:37663930</ref> | ||
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 8hi1" style="background-color:#fffaf0;"></div> | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
Streptococcus thermophilus Cas1-Cas2- prespacer ternary complex
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