3qg6

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<StructureSection load='3qg6' size='340' side='right'caption='[[3qg6]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
<StructureSection load='3qg6' size='340' side='right'caption='[[3qg6]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3qg6]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QG6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QG6 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3qg6]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QG6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QG6 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3qg7|3qg7]]</div></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qg6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qg6 OCA], [https://pdbe.org/3qg6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qg6 RCSB], [https://www.ebi.ac.uk/pdbsum/3qg6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qg6 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qg6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qg6 OCA], [https://pdbe.org/3qg6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qg6 RCSB], [https://www.ebi.ac.uk/pdbsum/3qg6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qg6 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q9F6Z3_STAAU Q9F6Z3_STAAU]
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In the postantibiotic era, available treatment options for severe bacterial infections caused by methicillin-resistant Staphylococcus aureus have become limited. Therefore, new and innovative approaches are needed to combat such life-threatening infections. Virulence factor expression in S. aureus is regulated in a cell density-dependent manner using "quorum sensing," which involves generation and secretion of autoinducing peptides (AIPs) into the surrounding environment to activate a bacterial sensor kinase at a particular threshold concentration. Mouse monoclonal antibody AP4-24H11 was shown previously to blunt quorum sensing-mediated changes in gene expression in vitro and protect mice from a lethal dose of S. aureus by sequestering the AIP signal. We have elucidated the crystal structure of the AP4-24H11 Fab in complex with AIP-4 at 2.5 A resolution to determine its mechanism of ligand recognition. A key Glu(H95) provides much of the binding specificity through formation of hydrogen bonds with each of the four amide nitrogens in the AIP-4 macrocyclic ring. Importantly, these structural data give clues as to the interactions between the cognate staphylococcal AIP receptors AgrC and the AIPs, as AP4-24H11.AIP-4 binding recapitulates features that have been proposed for AgrC-AIP recognition. Additionally, these structural insights may enable the engineering of AIP cross-reactive antibodies or quorum quenching vaccines for use in active or passive immunotherapy for prevention or treatment of S. aureus infections.
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Structural Basis for Ligand Recognition and Discrimination of a Quorum-quenching Antibody.,Kirchdoerfer RN, Garner AL, Flack CE, Mee JM, Horswill AR, Janda KD, Kaufmann GF, Wilson IA J Biol Chem. 2011 May 13;286(19):17351-8. Epub 2011 Mar 23. PMID:21454495<ref>PMID:21454495</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3qg6" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Lk3 transgenic mice]]
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[[Category: Mus musculus]]
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[[Category: Janda, J D]]
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[[Category: Staphylococcus aureus]]
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[[Category: Kaufmann, G F]]
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[[Category: Janda JD]]
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[[Category: Kirchdoerfer, R N]]
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[[Category: Kaufmann GF]]
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[[Category: Wilson, I A]]
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[[Category: Kirchdoerfer RN]]
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[[Category: Aip4 binding]]
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[[Category: Wilson IA]]
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[[Category: Antigen binding]]
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[[Category: Immune system-inhibitor complex]]
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[[Category: Immunoglobulin fold]]
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[[Category: Secreted]]
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Revision as of 11:44, 14 March 2024

Structural Basis for Ligand Recognition and Discrimination of a Quorum Quenching Antibody

PDB ID 3qg6

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