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1r1c

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[[Image:1r1c.jpg|left|200px]]
[[Image:1r1c.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1r1c |SIZE=350|CAPTION= <scene name='initialview01'>1r1c</scene>, resolution 1.90&Aring;
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The line below this paragraph, containing "STRUCTURE_1r1c", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene>, <scene name='pdbligand=REP:(1,10+PHENANTHROLINE)-(TRI-CARBON+MONOXIDE)+RHENIUM+(I)'>REP</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= AZU OR PA4922 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 Pseudomonas aeruginosa])
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|DOMAIN=
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{{STRUCTURE_1r1c| PDB=1r1c | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1r1c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r1c OCA], [http://www.ebi.ac.uk/pdbsum/1r1c PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1r1c RCSB]</span>
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}}
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'''PSEUDOMONAS AERUGINOSA W48F/Y72F/H83Q/Y108W-AZURIN RE(PHEN)(CO)3(HIS107)'''
'''PSEUDOMONAS AERUGINOSA W48F/Y72F/H83Q/Y108W-AZURIN RE(PHEN)(CO)3(HIS107)'''
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[[Category: Gradinaru, C.]]
[[Category: Gradinaru, C.]]
[[Category: Miller, J E.]]
[[Category: Miller, J E.]]
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[[Category: blue-copper]]
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[[Category: Blue-copper]]
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[[Category: electron-transfer]]
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[[Category: Electron-transfer]]
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[[Category: epr]]
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[[Category: Epr]]
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[[Category: radical]]
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[[Category: Radical]]
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[[Category: rhenium]]
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[[Category: Rhenium]]
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[[Category: tunneling]]
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[[Category: Tunneling]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:57:23 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:21:55 2008''
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Revision as of 03:57, 3 May 2008

Image:1r1c.jpg

Template:STRUCTURE 1r1c

PSEUDOMONAS AERUGINOSA W48F/Y72F/H83Q/Y108W-AZURIN RE(PHEN)(CO)3(HIS107)


Overview

Near-UV irradiation of structurally characterized [Re(I)(CO)3(1,10-phenanthroline)(Q107H)](W48F/Y72F/H83Q/Y108W)AzM(II) [Az = Pseudomonas aeruginosa azurin, M = Cu, Zn]/[Co(NH3)5Cl]Cl2 produces a tryptophan radical (W108*) with unprecedented kinetic stability. After rapid formation (k = 2.8 x 106 s-1), the radical persists for more than 5 h at room temperature in the folded ReAzM(II) structure. The absorption spectrum of ReAz(W108*)M(II) exhibits maxima at 512 and 536 nm. Oxidation of K4[Mo(CN)8] by ReAz(W108*)Zn(II) places the W108*/W108 reduction potential in the protein above 0.8 V vs NHE.

About this Structure

1R1C is a Single protein structure of sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.

Reference

Spectroscopy and reactivity of a photogenerated tryptophan radical in a structurally defined protein environment., Miller JE, Gradinaru C, Crane BR, Di Bilio AJ, Wehbi WA, Un S, Winkler JR, Gray HB, J Am Chem Soc. 2003 Nov 26;125(47):14220-1. PMID:14624538 Page seeded by OCA on Sat May 3 06:57:23 2008

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