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| | <StructureSection load='3qyq' size='340' side='right'caption='[[3qyq]], [[Resolution|resolution]] 1.80Å' scene=''> | | <StructureSection load='3qyq' size='340' side='right'caption='[[3qyq]], [[Resolution|resolution]] 1.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3qyq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Toxgm Toxgm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QYQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QYQ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3qyq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Toxoplasma_gondii_ME49 Toxoplasma gondii ME49]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QYQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QYQ FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TGME49_118750 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=508771 TOXGM])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900003:sucrose'>PRD_900003</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Deoxyribose-phosphate_aldolase Deoxyribose-phosphate aldolase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.4 4.1.2.4] </span></td></tr> | + | |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qyq OCA], [https://pdbe.org/3qyq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qyq RCSB], [https://www.ebi.ac.uk/pdbsum/3qyq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qyq ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qyq OCA], [https://pdbe.org/3qyq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qyq RCSB], [https://www.ebi.ac.uk/pdbsum/3qyq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qyq ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/B9PVB4_TOXGV B9PVB4_TOXGV] |
| - | Parasites of the phylum Apicomplexa are highly successful pathogens of humans and animals world-wide. As obligate intracellular parasites, they have significant energy requirements for invasion and gliding motility that are supplied by various metabolic pathways. Aldolases have emerged as key enzymes involved in these pathways, and all apicomplexans express one or both of fructose 1,6-bisphosphate (F16BP) aldolase and 2-deoxyribose 5-phosphate (dR5P) aldolase (DERA). Intriguingly, Toxoplasma gondii, a highly successful apicomplexan parasite, expresses F16BP aldolase (TgALD1), d5RP aldolase (TgDERA), and a divergent dR5P aldolase-like protein (TgDPA) exclusively in the latent bradyzoite stage. While the importance of TgALD1 in glycolysis is well established and TgDERA is also likely to be involved in parasite metabolism, the detailed function of TgDPA remains elusive. To gain mechanistic insight into the function of different T. gondii aldolases, we first determined the crystal structures of TgALD1 and TgDPA. Structural analysis revealed that both aldolases adopt a TIM barrel fold accessorized with divergent secondary structure elements. Structural comparison of TgALD1 and TgDPA with members of their respective enzyme families revealed that while the active site residues are conserved in TgALD1, key catalytic residues are absent in TgDPA. Consistent with this observation, biochemical assays showed that while TgALD1 was active on F16BP, TgDPA was inactive on dR5P. Intriguingly, both aldolases are competent to bind polymerized actin in vitro. Altogether, structural and biochemical analyses of T. gondii aldolase and aldolase-like proteins reveal diverse functionalization of the classic TIM barrel aldolase fold.
| + | |
| - | | + | |
| - | Structural and functional divergence of the aldolase fold in Toxoplasma gondii.,Tonkin ML, Halavaty AS, Ramaswamy R, Ruan J, Igarashi M, Ngo HM, Boulanger MJ J Mol Biol. 2014 Oct 2. pii: S0022-2836(14)00518-X. doi:, 10.1016/j.jmb.2014.09.019. PMID:25284756<ref>PMID:25284756</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 3qyq" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Deoxyribose-phosphate aldolase]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Toxgm]] | + | [[Category: Toxoplasma gondii ME49]] |
| - | [[Category: Anderson, W F]] | + | [[Category: Anderson WF]] |
| - | [[Category: Structural genomic]]
| + | [[Category: Halavaty AS]] |
| - | [[Category: Halavaty, A S]] | + | [[Category: Igarashi M]] |
| - | [[Category: Igarashi, M]] | + | [[Category: Minasov G]] |
| - | [[Category: Minasov, G]] | + | [[Category: Ngo H]] |
| - | [[Category: Ngo, H]] | + | [[Category: Ruan J]] |
| - | [[Category: Ruan, J]] | + | [[Category: Shuvalova L]] |
| - | [[Category: Shuvalova, L]] | + | [[Category: Ueno A]] |
| - | [[Category: Ueno, A]] | + | |
| - | [[Category: Bradyzoite]]
| + | |
| - | [[Category: Brain cyst]]
| + | |
| - | [[Category: Chemotherapy]]
| + | |
| - | [[Category: Csgid]]
| + | |
| - | [[Category: Lyase]]
| + | |
| - | [[Category: Tim-barrel]]
| + | |
