3r3j

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Current revision (12:09, 14 March 2024) (edit) (undo)
 
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<StructureSection load='3r3j' size='340' side='right'caption='[[3r3j]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
<StructureSection load='3r3j' size='340' side='right'caption='[[3r3j]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3r3j]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Plaf7 Plaf7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R3J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3R3J FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3r3j]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum_3D7 Plasmodium falciparum 3D7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R3J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3R3J FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PF14_0286 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36329 PLAF7])</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Glutamate_dehydrogenase_(NAD(P)(+)) Glutamate dehydrogenase (NAD(P)(+))], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.1.3 1.4.1.3] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3r3j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r3j OCA], [https://pdbe.org/3r3j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3r3j RCSB], [https://www.ebi.ac.uk/pdbsum/3r3j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3r3j ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3r3j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r3j OCA], [https://pdbe.org/3r3j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3r3j RCSB], [https://www.ebi.ac.uk/pdbsum/3r3j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3r3j ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q8ILF7_PLAF7 Q8ILF7_PLAF7]
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Glutamate dehydrogenases (GDHs) play key roles in cellular redox, amino acid, and energy metabolism, thus representing potential targets for pharmacological interventions. Here we studied the functional network provided by the three known glutamate dehydrogenases of the malaria parasite Plasmodium falciparum. The recombinant production of the previously described PfGDH1 as hexahistidyl-tagged proteins was optimized. Additionally, PfGDH2 was cloned, recombinantly produced, and characterized. Like PfGDH1, PfGDH2 is an NADP(H)-dependent enzyme with a specific activity comparable to PfGDH1 but with slightly higher K(m) values for its substrates. The three-dimensional structure of hexameric PfGDH2 was solved to 3.1A resolution. The overall structure shows high similarity with PfGDH1 but with significant differences occurring at the subunit interface. As in mammalian GDH1, in PfGDH2 the subunit-subunit interactions are mainly assisted by hydrogen bonds and hydrophobic interactions, whereas in PfGDH1 these contacts are mediated by networks of salt bridges and hydrogen bonds. In accordance with this, the known bovine GDH inhibitors hexachlorophene, GW5074, and bithionol were more effective on PfGDH2 than on PfGDH1. Subcellular localization was determined for all three plasmodial GDHs by fusion with the green fluorescent protein. Based on our data, PfGDH1 and PfGDH3 are cytosolic proteins whereas PfGDH2 clearly localizes to the apicoplast, a plastid-like organelle specific for apicomplexan parasites. This study provides new insights into the structure and function of GDH isoenzymes of P. falciparum, which represent potential targets for the development of novel antimalarial drugs.
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Biochemical and structural characterization of Plasmodium falciparum glutamate dehydrogenase 2.,Zocher K, Fritz-Wolf K, Kehr S, Fischer M, Rahlfs S, Becker K Mol Biochem Parasitol. 2012 Feb 9. PMID:22342964<ref>PMID:22342964</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3r3j" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
*[[Glutamate dehydrogenase 3D structures|Glutamate dehydrogenase 3D structures]]
*[[Glutamate dehydrogenase 3D structures|Glutamate dehydrogenase 3D structures]]
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== References ==
 
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<references/>
 
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Plaf7]]
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[[Category: Plasmodium falciparum 3D7]]
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[[Category: Becker, K]]
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[[Category: Becker K]]
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[[Category: Fritz-Wolf, K]]
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[[Category: Fritz-Wolf K]]
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[[Category: Kehr, S]]
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[[Category: Kehr S]]
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[[Category: Rahlfs, S]]
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[[Category: Rahlfs S]]
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[[Category: Zocher, K]]
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[[Category: Zocher K]]
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[[Category: Apicoplast]]
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[[Category: Oxidoreductase]]
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[[Category: Plasmodium falciparum]]
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[[Category: Rossmann fold]]
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Current revision

Kinetic and structural characterization of Plasmodium falciparum glutamate dehydrogenase 2

PDB ID 3r3j

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