|
|
| Line 3: |
Line 3: |
| | <StructureSection load='3rkr' size='340' side='right'caption='[[3rkr]], [[Resolution|resolution]] 2.42Å' scene=''> | | <StructureSection load='3rkr' size='340' side='right'caption='[[3rkr]], [[Resolution|resolution]] 2.42Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3rkr]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Uncultured_bacterium_bio5 Uncultured bacterium bio5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RKR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RKR FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3rkr]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Uncultured_bacterium_Bio5 Uncultured bacterium Bio5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RKR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RKR FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.42Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/3-oxoacyl-[acyl-carrier-protein]_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.100 1.1.1.100] </span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rkr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rkr OCA], [https://pdbe.org/3rkr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rkr RCSB], [https://www.ebi.ac.uk/pdbsum/3rkr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rkr ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rkr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rkr OCA], [https://pdbe.org/3rkr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rkr RCSB], [https://www.ebi.ac.uk/pdbsum/3rkr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rkr ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/B2BKB1_9BACT B2BKB1_9BACT] |
| - | In Pseudomonas aeruginosa, the expression of a number of virulence factors, as well as biofilm formation, are controlled by quorum sensing (QS). N-Acylhomoserine lactones (AHLs) are an important class of signaling molecules involved in bacterial QS and in many pathogenic bacteria infection and host colonization are AHL-dependent. The AHL signaling molecules are subject to inactivation mainly by hydrolases (Enzyme Commission class number EC 3) (i.e. N-acyl-homoserine lactonases and N-acyl-homoserine-lactone acylases). Only little is known on quorum quenching mechanisms of oxidoreductases (EC 1). Here we report on the identification and structural characterization of the first NADP-dependent short-chain dehydrogenase/reductase (SDR) involved in inactivation of N-(3-oxo-dodecanoyl)-L-homoserine lactone (3-oxo-C(12)-HSL) and derived from a metagenome library. The corresponding gene was isolated from a soil metagenome and designated bpiB09. Heterologous expression and crystallographic studies established BpiB09 as an NADP-dependent reductase. Although AHLs are probably not the native substrate of this metagenome-derived enzyme, its expression in P. aeruginosa PAO1 resulted in significantly reduced pyocyanin production, decreased motility, poor biofilm formation and absent paralysis of Caenorhabditis elegans. Furthermore, a genome-wide transcriptome study suggested that the level of lasI and rhlI transcription together with 36 well known QS regulated genes was significantly (>/=10-fold) affected in P. aeruginosa strains expressing the bpiB09 gene in pBBR1MCS-5. Thus AHL oxidoreductases could be considered as potent tools for the development of quorum quenching strategies.
| + | |
| - | | + | |
| - | A novel metagenomic short-chain dehydrogenase/reductase attenuates Pseudomonas aeruginosa biofilm formation and virulence on Caenorhabditis elegans.,Bijtenhoorn P, Mayerhofer H, Muller-Dieckmann J, Utpatel C, Schipper C, Hornung C, Szesny M, Grond S, Thurmer A, Brzuszkiewicz E, Daniel R, Dierking K, Schulenburg H, Streit WR PLoS One. 2011;6(10):e26278. Epub 2011 Oct 26. PMID:22046268<ref>PMID:22046268</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 3rkr" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Uncultured bacterium bio5]] | + | [[Category: Uncultured bacterium Bio5]] |
| - | [[Category: Bijtenhoorn, P]] | + | [[Category: Bijtenhoorn P]] |
| - | [[Category: Mayerhofer, H]] | + | [[Category: Mayerhofer H]] |
| - | [[Category: Mueller-Dieckmann, J]] | + | [[Category: Mueller-Dieckmann J]] |
| - | [[Category: Streit, W R]] | + | [[Category: Streit WR]] |
| - | [[Category: Oxidoreductase]]
| + | |
| - | [[Category: Rossmann fold]]
| + | |
