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| | <StructureSection load='3rli' size='340' side='right'caption='[[3rli]], [[Resolution|resolution]] 1.85Å' scene=''> | | <StructureSection load='3rli' size='340' side='right'caption='[[3rli]], [[Resolution|resolution]] 1.85Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3rli]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bac25 Bac25]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RLI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RLI FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3rli]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_sp._H-257 Bacillus sp. H-257]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RLI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RLI FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>, <scene name='pdbligand=PMS:PHENYLMETHANESULFONIC+ACID'>PMS</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.854Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Acylglycerol_lipase Acylglycerol lipase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.23 3.1.1.23] </span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>, <scene name='pdbligand=PMS:PHENYLMETHANESULFONIC+ACID'>PMS</scene></td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rli OCA], [https://pdbe.org/3rli PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rli RCSB], [https://www.ebi.ac.uk/pdbsum/3rli PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rli ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rli OCA], [https://pdbe.org/3rli PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rli RCSB], [https://www.ebi.ac.uk/pdbsum/3rli PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rli ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/MGLP_BAC25 MGLP_BAC25]] Hydrolyzes monoacylglycerols, with the highest activity occurring with 1-monolauroylglycerol.
| + | [https://www.uniprot.org/uniprot/MGLP_BAC25 MGLP_BAC25] Hydrolyzes monoacylglycerols, with the highest activity occurring with 1-monolauroylglycerol. |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | Monoacylglycerol lipases (MGLs) catalyse the hydrolysis of monoacylglycerol into free fatty acid and glycerol. MGLs have been identified throughout all genera of life and have adopted different substrate specificities depending on their physiological role. In humans, MGL plays an integral part in lipid metabolism affecting energy homeostasis, signalling processes and cancer cell progression. In bacteria, MGLs degrade short-chain monoacylglycerols which are otherwise toxic to the organism. We report the crystal structures of MGL from the bacterium Bacillus sp. H257 (bMGL) in its free form at 1.2A and in complex with phenylmethylsulfonyl fluoride at 1.8A resolution. In both structures, bMGL adopts an alpha/beta hydrolase fold with a cap in an open conformation. Access to the active site residues, which were unambiguously identified from the protein structure, is facilitated by two different channels. The larger channel constitutes the highly hydrophobic substrate binding pocket with enough room to accommodate monoacylglycerol. The other channel is rather small and resembles the proposed glycerol exit hole in human MGL. Molecular dynamics simulation of bMGL yielded open and closed states of the entrance channel and the glycerol exit hole. Despite differences in the number of residues, secondary structure elements, and low sequence identity in the cap region, this first structure of a bacterial MGL reveals striking structural conservation of the overall cap architecture in comparison with human MGL. Thus it provides insight into the structural conservation of the cap amongst MGLs throughout evolution and provides a framework for rationalising substrate specificities in each organism.
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| - | The structure of monoacylglycerol lipase from Bacillus sp. H257 reveals unexpected conservation of the cap architecture between bacterial and human enzymes.,Rengachari S, Bezerra GA, Riegler-Berket L, Gruber CC, Sturm C, Taschler U, Boeszoermenyi A, Dreveny I, Zimmermann R, Gruber K, Oberer M Biochim Biophys Acta. 2012 Jul;1821(7):1012-21. Epub 2012 Apr 27. PMID:22561231<ref>PMID:22561231</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
| + | |
| - | <div class="pdbe-citations 3rli" style="background-color:#fffaf0;"></div>
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| | | | |
| | ==See Also== | | ==See Also== |
| | *[[Lipase 3D Structures|Lipase 3D Structures]] | | *[[Lipase 3D Structures|Lipase 3D Structures]] |
| - | == References == | |
| - | <references/> | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Acylglycerol lipase]] | + | [[Category: Bacillus sp. H-257]] |
| - | [[Category: Bac25]]
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| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Bezerra, G A]] | + | [[Category: Bezerra GA]] |
| - | [[Category: Gruber, K]] | + | [[Category: Gruber K]] |
| - | [[Category: Oberer, M]] | + | [[Category: Oberer M]] |
| - | [[Category: Rengachari, S]] | + | [[Category: Rengachari S]] |
| - | [[Category: Alpha/beta hydrolase]]
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| - | [[Category: Hydrolase]]
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