3s60

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (12:47, 14 March 2024) (edit) (undo)
 
Line 3: Line 3:
<StructureSection load='3s60' size='340' side='right'caption='[[3s60]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
<StructureSection load='3s60' size='340' side='right'caption='[[3s60]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[3s60]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"planktothrix_agardhii"_emend._suda_et_al._2002 "planktothrix agardhii" emend. suda et al. 2002]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S60 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3S60 FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[3s60]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Planktothrix_agardhii Planktothrix agardhii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S60 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3S60 FirstGlance]. <br>
-
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3s5v|3s5v]], [[3s5x|3s5x]], [[3obl|3obl]]</div></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
-
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">OAA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1160 "Planktothrix agardhii" emend. Suda et al. 2002])</td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3s60 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s60 OCA], [https://pdbe.org/3s60 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3s60 RCSB], [https://www.ebi.ac.uk/pdbsum/3s60 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3s60 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3s60 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s60 OCA], [https://pdbe.org/3s60 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3s60 RCSB], [https://www.ebi.ac.uk/pdbsum/3s60 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3s60 ProSAT]</span></td></tr>
</table>
</table>
-
<div style="background-color:#fffaf0;">
+
== Function ==
-
== Publication Abstract from PubMed ==
+
[https://www.uniprot.org/uniprot/LEC1_PLAAG LEC1_PLAAG] Lectin specific for high mannose N-glycans, recognizes the branched moiety of these glycans. Does not recognize other types of N-glycans or monosaccharides. Agglutinates trypsin-treated rabbit erythrocytes. Does not require divalent cations for activity. Inhibits HIV replication in MT4 cells with an EC(50) of 45 nM. Binds to the HIV envelope glycoprotein gp120.<ref>PMID:10817903</ref> <ref>PMID:17314091</ref>
-
The cyanobacterial Oscillatory Agardhii agglutinin (OAA) is a recently discovered HIV-inactivating lectin that interacts with high-mannose sugars. Nuclear magnetic resonance (NMR) binding studies between OAA and alpha3,alpha6-mannopentaose (Manalpha(1-3)[Manalpha(1-3)[Manalpha(1-6)]Manalpha(1-6)]Man), the branched core unit of Man-9, revealed two binding sites at opposite ends of the protein, exhibiting essentially identical affinities. Atomic details of the specific protein-sugar contacts in the recognition loops of OAA were delineated in the high-resolution crystal structures of free and glycan-complexed protein. No major changes in the overall protein structure are induced by carbohydrate binding, with essentially identical apo- and sugar-bound conformations in binding site 1. A single peptide bond flip at W77-G78 is seen in binding site 2. Our combined NMR and crystallographic results provide structural insights into the mechanism by which OAA specifically recognizes the branched Man-9 core, distinctly different from the recognition of the D1 and D3 arms at the nonreducing end of high-mannose carbohydrates by other antiviral lectins.
+
-
 
+
-
Structural basis of the anti-HIV activity of the cyanobacterial Oscillatoria Agardhii agglutinin.,Koharudin LM, Gronenborn AM Structure. 2011 Aug 10;19(8):1170-81. PMID:21827952<ref>PMID:21827952</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 3s60" style="background-color:#fffaf0;"></div>
+
==See Also==
==See Also==
Line 24: Line 16:
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Planktothrix agardhii emend. suda et al. 2002]]
 
[[Category: Large Structures]]
[[Category: Large Structures]]
-
[[Category: Gronenborn, A M]]
+
[[Category: Planktothrix agardhii]]
-
[[Category: Koharudin, L M.I]]
+
[[Category: Gronenborn AM]]
-
[[Category: Anti-hiv lectin]]
+
[[Category: Koharudin LMI]]
-
[[Category: Beta barrel like fold]]
+
-
[[Category: Carbohydrate]]
+
-
[[Category: Protein binding]]
+

Current revision

Structure of the cyanobacterial Oscillatoria Agardhii Agglutinin (OAA) in free state obtained at 25 degree Celsius

PDB ID 3s60

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3s60"
Personal tools