3sj9

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crystal structure of the C147A mutant 3C of CVA16 in complex with FAGLRQAVTQ peptide

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 <StructureSection load='3sj9' size='340' side='right'caption='[[3sj9]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3sj9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cv-a16 Cv-a16]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SJ9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SJ9 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3sj9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Coxsackievirus_A16 Coxsackievirus A16]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SJ9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SJ9 FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1cqq|1cqq]], [[2zty|2zty]], [[1l1n|1l1n]], [[3sj8|3sj8]], [[3sji|3sji]], [[3sjk|3sjk]], [[3sjo|3sjo]]</div></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.399&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">3C ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=31704 CV-A16])</td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Picornain_3C Picornain 3C], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.28 3.4.22.28] </span></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3sj9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sj9 OCA], [https://pdbe.org/3sj9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3sj9 RCSB], [https://www.ebi.ac.uk/pdbsum/3sj9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3sj9 ProSAT]</span></td></tr>
 </table>
-<div style="background-color:#fffaf0;">
+== Function ==
-== Publication Abstract from PubMed ==
+[https://www.uniprot.org/uniprot/C8CIL7_9ENTO C8CIL7_9ENTO]
-Enterovirus 71 (EV71) and coxsackievirus A16 (CVA16) are the major causative agents of hand foot and mouth disease (HFMD) which is prevalent in Asia. Thus far there are no prophylactic or therapeutic measures against HFMD. The 3C proteases from EV71 and CVA16 play important roles in viral replication and are therefore ideal drug targets. By using biochemical, mutational and structural approaches, we broadly characterized both proteases. A series of high-resolution structures of the free or substrate-bound enzymes were solved. These structures, together with our cleavage specificity assay, well explain the marked substrate preferences of both proteases for particular P4, P1 and P1' residue types, as well as their relative malleability for P2 amino acid. More importantly, the complex structures of EV71 and CVA16 3Cs with Rupintrivir, a specific human rhinovirus (HRV) 3C protease inhibitor, were solved. These structures reveal a half-closed S2 subsite and a size-reduced S1' subsite that limit the access of the P1' group of Rupitrivir to both enzymes, explaining the reported low inhibition activity of the compound toward EV71 and CVA16. In conclusion, the detailed characterization of both proteases in this study could direct us for a proposal of rational design of EV71/CVA16 3C inhibitors.
-Enterovirus 71 and Coxsackievirus A16 3C proteases: binding to Rupintrivir and their substrate, and anti-HFMD drug design.,Lu G, Qi J, Chen Z, Xu X, Gao F, Lin D, Qian W, Liu H, Jiang H, Yan J, Gao GF J Virol. 2011 Jul 27. PMID:21795339<ref>PMID:21795339</ref>
-From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-</div>
-<div class="pdbe-citations 3sj9" style="background-color:#fffaf0;"></div>
 ==See Also==
 *[[Virus protease 3D structures|Virus protease 3D structures]]
-== References ==
-<references/>
 __TOC__
 </StructureSection>
-[[Category: Cv-a16]]
+[[Category: Coxsackievirus A16]]
 [[Category: Large Structures]]
-[[Category: Picornain 3C]]
+[[Category: Chen Z]]
-[[Category: Chen, Z]]
+[[Category: Gao F]]
-[[Category: Gao, F]]
+[[Category: Gao GF]]
-[[Category: Gao, G F]]
+[[Category: Jiang H]]
-[[Category: Jiang, H]]
+[[Category: Lin D]]
-[[Category: Lin, D]]
+[[Category: Liu H]]
-[[Category: Liu, H]]
+[[Category: Lu G]]
-[[Category: Lu, G]]
+[[Category: Qi J]]
-[[Category: Qi, J]]
+[[Category: Qian W]]
-[[Category: Qian, W]]
+[[Category: Xu X]]
-[[Category: Xu, X]]
+[[Category: Yan J]]
-[[Category: Yan, J]]
-[[Category: Binding to its substrate-peptide]]
-[[Category: Chymotrypsin-like fold]]
-[[Category: Hydrolase]]
-[[Category: Protease]]

3sj9

From Proteopedia

Current revision

crystal structure of the C147A mutant 3C of CVA16 in complex with FAGLRQAVTQ peptide

Structural highlights

Function

See Also

Contents

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