3t6d

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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3t6d]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Blastochloris_viridis Blastochloris viridis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T6D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T6D FirstGlance]. <br>
<table><tr><td colspan='2'>[[3t6d]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Blastochloris_viridis Blastochloris viridis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T6D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T6D FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BCB:BACTERIOCHLOROPHYLL+B'>BCB</scene>, <scene name='pdbligand=BPB:BACTERIOPHEOPHYTIN+B'>BPB</scene>, <scene name='pdbligand=DGA:DIACYL+GLYCEROL'>DGA</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=HTH:(2S,3R)-HEPTANE-1,2,3-TRIOL'>HTH</scene>, <scene name='pdbligand=HTO:HEPTANE-1,2,3-TRIOL'>HTO</scene>, <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene>, <scene name='pdbligand=MQ9:MENAQUINONE-9'>MQ9</scene>, <scene name='pdbligand=NS5:15-CIS-1,2-DIHYDRONEUROSPORENE'>NS5</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UQ9:UBIQUINONE-9'>UQ9</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BCB:BACTERIOCHLOROPHYLL+B'>BCB</scene>, <scene name='pdbligand=BPB:BACTERIOPHEOPHYTIN+B'>BPB</scene>, <scene name='pdbligand=DGA:DIACYL+GLYCEROL'>DGA</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=HTH:(2S,3R)-HEPTANE-1,2,3-TRIOL'>HTH</scene>, <scene name='pdbligand=HTO:HEPTANE-1,2,3-TRIOL'>HTO</scene>, <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene>, <scene name='pdbligand=MQ9:MENAQUINONE-9'>MQ9</scene>, <scene name='pdbligand=NS5:15-CIS-1,2-DIHYDRONEUROSPORENE'>NS5</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UQ9:UBIQUINONE-9'>UQ9</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3t6e|3t6e]], [[2wjm|2wjm]], [[2wjn|2wjn]], [[2jbl|2jbl]], [[2i5n|2i5n]], [[1vrn|1vrn]], [[1dxr|1dxr]], [[1prc|1prc]]</div></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t6d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t6d OCA], [https://pdbe.org/3t6d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t6d RCSB], [https://www.ebi.ac.uk/pdbsum/3t6d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t6d ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t6d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t6d OCA], [https://pdbe.org/3t6d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t6d RCSB], [https://www.ebi.ac.uk/pdbsum/3t6d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t6d ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[https://www.uniprot.org/uniprot/B8Y5U8_BLAVI B8Y5U8_BLAVI]] The reaction center of purple bacteria contains a tightly bound cytochrome molecule which re-reduces the photo oxidized primary electron donor.[PIRNR:PIRNR000017]
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[https://www.uniprot.org/uniprot/CYCR_BLAVI CYCR_BLAVI] The reaction center of purple bacteria contains a tightly bound cytochrome molecule which re-reduces the photo oxidized primary electron donor.<ref>PMID:10736158</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Newly determined crystal structures of the photosynthetic reaction centre (RC) from two substrains of the non-sulphur purple bacterium Blastochloris viridis strain DSM 133, together with analysis of their gene sequences, has revealed intra-species evolutionary changes over a time period of 14 years. Over one hundred point mutations were identified between these two substrains in the four genes encoding the protein subunits of the RC, of which about one fifth resulted in a total of 16 amino acid changes. The most interesting difference was in the M subunit where the change from leucine to glycine in the carotenoid binding pocket allowed dihydroneurosporene to adopt a more sterically favoured conformation and similar to the carotenoid conformation found in other related reaction centres. Our results together with a high rate of mutations in laboratory bacterial cultures described recently [Perfeito, Fernandes, Mota and Gordo (2007) Science 317, 813-815] suggest that bacteria evolve faster than has been generally recognised. The possibility that amino acid changes occur within protein sequences, without exhibiting any immediately observable phenotype, should be taken into account in studies that involve long-term continuous growth of pure bacterial cultures. The Blastochloris viridis RC is often studied with sophisticated biophysical techniques and changes such as those described here may well affect their outcome. In other words, there is a danger that laboratory-to-laboratory variation could well be due to different groups not realising that they are actually working with slightly different proteins. A way around this problem is suggested.
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New Insights into the Structure of the Reaction Centre from Blastochloris viridis: Evolution in the Laboratory.,Roszak AW, Moulisova V, Reksodipuro AD, Gardiner AT, Fujii R, Hashimoto H, Isaacs NW, Cogdell RJ Biochem J. 2011 Nov 4. PMID:22054235<ref>PMID:22054235</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3t6d" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
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[[Category: Blastochloris viridis]]
[[Category: Blastochloris viridis]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Cogdell, R J]]
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[[Category: Cogdell RJ]]
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[[Category: Gardiner, A T]]
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[[Category: Gardiner AT]]
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[[Category: Isaacs, N W]]
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[[Category: Isaacs NW]]
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[[Category: Roszak, A W]]
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[[Category: Roszak AW]]
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[[Category: Electron transport]]
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[[Category: Evolution]]
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[[Category: Genetic drift]]
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[[Category: Photosynthesis]]
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[[Category: Photosynthetic membrane]]
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[[Category: Photosynthetic reaction center]]
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[[Category: Pigment-protein complex]]
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Revision as of 13:18, 14 March 2024

Crystal Structure of the Reaction Centre from Blastochloris viridis strain DSM 133 (ATCC 19567) substrain-08

PDB ID 3t6d

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