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| | <StructureSection load='3tf5' size='340' side='right'caption='[[3tf5]], [[Resolution|resolution]] 2.30Å' scene=''> | | <StructureSection load='3tf5' size='340' side='right'caption='[[3tf5]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3tf5]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TF5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TF5 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3tf5]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TF5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TF5 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3prj|3prj]], [[3ptz|3ptz]], [[2q3e|2q3e]], [[2qg4|2qg4]]</div></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NM_003359, UGDH ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
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| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/UDP-glucose_6-dehydrogenase UDP-glucose 6-dehydrogenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.22 1.1.1.22] </span></td></tr>
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| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tf5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tf5 OCA], [https://pdbe.org/3tf5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tf5 RCSB], [https://www.ebi.ac.uk/pdbsum/3tf5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tf5 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tf5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tf5 OCA], [https://pdbe.org/3tf5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tf5 RCSB], [https://www.ebi.ac.uk/pdbsum/3tf5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tf5 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/UGDH_HUMAN UGDH_HUMAN]] Involved in the biosynthesis of glycosaminoglycans; hyaluronan, chondroitin sulfate, and heparan sulfate.
| + | [https://www.uniprot.org/uniprot/UGDH_HUMAN UGDH_HUMAN] Involved in the biosynthesis of glycosaminoglycans; hyaluronan, chondroitin sulfate, and heparan sulfate. |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | UDP-alpha-D-xylose (UDX) acts as a feedback inhibitor of human UDP-alpha-D-glucose 6-dehydrogenase (hUGDH) by activating an unusual allosteric switch, the Thr131 loop. UDX binding induces the Thr131 loop to translate ~5 A through the protein core, changing packing interactions and rotating a helix (alpha6(136-144)) to favor the formation of an inactive hexameric complex. But how does to conformational change occur given the steric packing constraints of the protein core? To answer this question, we deleted Val132 from the Thr131 loop to approximate an intermediate state in the allosteric transition. The 2.3 A resolution crystal structure of the deletion construct (Delta132) reveals an open conformation that relaxes steric constraints and facilitates repacking of the protein core. Sedimentation velocity studies show that the open conformation stabilizes the Delta132 construct as a hexamer with point group symmetry 32, similar to that of the active complex. In contrast, the UDX-inhibited enzyme forms a lower-symmetry, horseshoe-shaped hexameric complex. We show that the Delta132 and UDX-inhibited structures have similar hexamer-building interfaces, suggesting that the hinge-bending motion represents a path for the allosteric transition between the different hexameric states. On the basis of (i) main chain flexibility and (ii) a model of the conformational change, we propose that hinge bending can occur as a concerted motion between adjacent subunits in the high-symmetry hexamer. We combine these results in a structurally detailed model for allosteric feedback inhibition and substrate--product exchange during the catalytic cycle.
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| - | | + | |
| - | Conformational flexibility in the allosteric regulation of human UDP-alpha-D-glucose 6-dehydrogenase.,Sennett NC, Kadirvelraj R, Wood ZA Biochemistry. 2011 Nov 8;50(44):9651-63. Epub 2011 Oct 13. PMID:21961565<ref>PMID:21961565</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 3tf5" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: UDP-glucose 6-dehydrogenase]]
| + | [[Category: Sennett NC]] |
| - | [[Category: Sennett, N C]] | + | [[Category: Wood ZA]] |
| - | [[Category: Wood, Z A]] | + | |
| - | [[Category: Feedback inhibition]]
| + | |
| - | [[Category: Nad binding protein]]
| + | |
| - | [[Category: Nucleotide sugar dehydrogenase]]
| + | |
| - | [[Category: Oxidizes udp-glucose to udp-glucuronic acid]]
| + | |
| - | [[Category: Oxidoreductase]]
| + | |
| - | [[Category: Rossmann fold]]
| + | |
