1r2z

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[[Image:1r2z.gif|left|200px]]
[[Image:1r2z.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1r2z |SIZE=350|CAPTION= <scene name='initialview01'>1r2z</scene>, resolution 1.63&Aring;
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The line below this paragraph, containing "STRUCTURE_1r2z", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=UMP:2&#39;-DEOXYURIDINE+5&#39;-MONOPHOSPHATE'>UMP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= mutm ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1422 Geobacillus stearothermophilus])
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-->
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|DOMAIN=
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{{STRUCTURE_1r2z| PDB=1r2z | SCENE= }}
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|RELATEDENTRY=[[1l1t|1L1T]], [[1l1z|1L1Z]], [[1l2b|1L2B]], [[1l2c|1L2C]], [[1l2d|1L2D]], [[1r2y|1R2Y]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1r2z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r2z OCA], [http://www.ebi.ac.uk/pdbsum/1r2z PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1r2z RCSB]</span>
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}}
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'''MutM (Fpg) bound to 5,6-dihydrouracil (DHU) containing DNA'''
'''MutM (Fpg) bound to 5,6-dihydrouracil (DHU) containing DNA'''
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[[Category: Fromme, J C.]]
[[Category: Fromme, J C.]]
[[Category: Verdine, G L.]]
[[Category: Verdine, G L.]]
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[[Category: dna glycosylase]]
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[[Category: Dna glycosylase]]
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[[Category: dna repair]]
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[[Category: Dna repair]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:00:48 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:22:30 2008''
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Revision as of 04:00, 3 May 2008

Image:1r2z.gif

Template:STRUCTURE 1r2z

MutM (Fpg) bound to 5,6-dihydrouracil (DHU) containing DNA


Overview

MutM is a bacterial DNA glycosylase that removes the mutagenic lesion 8-oxoguanine (oxoG) from duplex DNA. The means of oxoG recognition by MutM (also known as Fpg) is of fundamental interest, in light of the vast excess of normal guanine bases present in genomic DNA. The crystal structure of a recognition-competent but catalytically inactive version of MutM in complex with oxoG-containing DNA reveals the structural basis for recognition. MutM binds the oxoG nucleoside in the syn glycosidic configuration and distinguishes oxoG from guanine by reading out the protonation state of the N7 atom. The segment of MutM principally responsible for oxoG recognition is a flexible loop, suggesting that conformational mobility influences lesion recognition and catalysis. Furthermore, the structure of MutM in complex with DNA containing an alternative substrate, dihydrouracil, demonstrates how MutM is able to recognize lesions other than oxoG.

About this Structure

1R2Z is a Single protein structure of sequence from Geobacillus stearothermophilus. Full crystallographic information is available from OCA.

Reference

DNA lesion recognition by the bacterial repair enzyme MutM., Fromme JC, Verdine GL, J Biol Chem. 2003 Dec 19;278(51):51543-8. Epub 2003 Oct 1. PMID:14525999 Page seeded by OCA on Sat May 3 07:00:48 2008

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OCA

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