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| | <StructureSection load='3ueq' size='340' side='right'caption='[[3ueq]], [[Resolution|resolution]] 1.85Å' scene=''> | | <StructureSection load='3ueq' size='340' side='right'caption='[[3ueq]], [[Resolution|resolution]] 1.85Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3ueq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"neisseria_polysacchareae"_riou_et_al._1983 "neisseria polysacchareae" riou et al. 1983]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UEQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UEQ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3ueq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Neisseria_polysaccharea Neisseria polysaccharea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UEQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UEQ FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=OTU:3-O-ALPHA-D-GLUCOPYRANOSYL-D-FRUCTOSE'>OTU</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1g5a|1g5a]], [[1jgi|1jgi]], [[1jg9|1jg9]], [[1mw0|1mw0]], [[1s46|1s46]]</div></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=OTU:3-O-ALPHA-D-GLUCOPYRANOSYL-D-FRUCTOSE'>OTU</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PRD_900047:beta-turanose'>PRD_900047</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ams ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=489 "Neisseria polysacchareae" Riou et al. 1983])</td></tr>
| + | |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Amylosucrase Amylosucrase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.4 2.4.1.4] </span></td></tr> | + | |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ueq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ueq OCA], [https://pdbe.org/3ueq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ueq RCSB], [https://www.ebi.ac.uk/pdbsum/3ueq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ueq ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ueq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ueq OCA], [https://pdbe.org/3ueq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ueq RCSB], [https://www.ebi.ac.uk/pdbsum/3ueq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ueq ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/AMYS_NEIPO AMYS_NEIPO]] Catalyzes the synthesis of alpha-glucan from sucrose. Catalyzes, in addition, sucrose hydrolysis, maltose and maltotriose synthesis by successive transfers of the glucosyl moiety of sucrose onto the released glucose, and finally turanose and trehalulose synthesis, these two sucrose isomers being obtained by glucosyl transfer onto fructose.<ref>PMID:9882648</ref>
| + | [https://www.uniprot.org/uniprot/AMYS_NEIPO AMYS_NEIPO] Catalyzes the synthesis of alpha-glucan from sucrose. Catalyzes, in addition, sucrose hydrolysis, maltose and maltotriose synthesis by successive transfers of the glucosyl moiety of sucrose onto the released glucose, and finally turanose and trehalulose synthesis, these two sucrose isomers being obtained by glucosyl transfer onto fructose.<ref>PMID:9882648</ref> |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | Amylosucrases are sucrose-utilizing alpha-transglucosidases that naturally catalyze the synthesis of alpha-glucans, linked exclusively through alpha1,4-linkages. Side products and in particular sucrose isomers such as turanose and trehalulose are also produced by these enzymes. Here, we report the first structural and biophysical characterization of the most thermostable amylosucrase identified so far, the amylosucrase from Deinoccocus geothermalis (DgAS). The three-dimensional structure revealed a homodimeric quaternary organization, never reported before for other amylosucrases. A sequence signature of dimerization was identified from the analysis of the dimer interface and sequence alignments. By rigidifying the DgAS structure, the quaternary organization is likely to participate in the enhanced thermal stability of the protein. Amylosucrase specificity with respect to sucrose isomer formation (turanose or trehalulose) was also investigated. We report the first structures of the amylosucrases from Deinococcus geothermalis and Neisseria polysaccharea in complex with turanose. In the amylosucrase from N. polysaccharea (NpAS), key residues were found to force the fructosyl moiety to bind in an open state with the O3' ideally positioned to explain the preferential formation of turanose by NpAS. Such residues are either not present or not similarly placed in DgAS. As a consequence, DgAS binds the furanoid tautomers of fructose through a weak network of interactions to enable turanose formation. Such topology at subsite +1 is likely favoring other possible fructose binding modes in agreement with the higher amount of trehalulose formed by DgAS. Our findings help to understand the inter-relationships between amylosucrase structure, flexibility, function, and stability and provide new insight for amylosucrase design.
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| - | | + | |
| - | Structural Investigation of the Thermostability and Product Specificity of Amylosucrase from the Bacterium Deinococcus geothermalis.,Guerin F, Barbe S, Pizzut-Serin S, Potocki-Veronese G, Guieysse D, Guillet V, Monsan P, Mourey L, Remaud-Simeon M, Andre I, Tranier S J Biol Chem. 2012 Feb 24;287(9):6642-54. Epub 2011 Dec 29. PMID:22210773<ref>PMID:22210773</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 3ueq" style="background-color:#fffaf0;"></div>
| + | |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Neisseria polysacchareae riou et al. 1983]] | |
| - | [[Category: Amylosucrase]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Andre, I]] | + | [[Category: Neisseria polysaccharea]] |
| - | [[Category: Guerin, F]] | + | [[Category: Andre I]] |
| - | [[Category: Guillet, V]] | + | [[Category: Guerin F]] |
| - | [[Category: Mourey, L]] | + | [[Category: Guillet V]] |
| - | [[Category: Pizzut-Serin, S]] | + | [[Category: Mourey L]] |
| - | [[Category: Potocki-Veronese, G]] | + | [[Category: Pizzut-Serin S]] |
| - | [[Category: Remaud-Simeon, M]] | + | [[Category: Potocki-Veronese G]] |
| - | [[Category: Tranier, S]] | + | [[Category: Remaud-Simeon M]] |
| - | [[Category: Amylose synthesis]]
| + | [[Category: Tranier S]] |
| - | [[Category: Beta/alpha-barrel]]
| + | |
| - | [[Category: Carbohydrate]]
| + | |
| - | [[Category: Glucosyltransferase]]
| + | |
| - | [[Category: Glycoside hydrolase]]
| + | |
| - | [[Category: Sucrose isomerization]]
| + | |
| - | [[Category: Transferase]]
| + | |
