3uv9

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Current revision (14:09, 14 March 2024) (edit) (undo)
 
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<StructureSection load='3uv9' size='340' side='right'caption='[[3uv9]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
<StructureSection load='3uv9' size='340' side='right'caption='[[3uv9]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3uv9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Macmu Macmu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UV9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UV9 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3uv9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Macaca_mulatta Macaca mulatta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UV9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UV9 FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2lm3|2lm3]]</div></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.549&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TRIM5 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9544 MACMU])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uv9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uv9 OCA], [https://pdbe.org/3uv9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uv9 RCSB], [https://www.ebi.ac.uk/pdbsum/3uv9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uv9 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uv9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uv9 OCA], [https://pdbe.org/3uv9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uv9 RCSB], [https://www.ebi.ac.uk/pdbsum/3uv9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uv9 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[https://www.uniprot.org/uniprot/TRIM5_MACMU TRIM5_MACMU]] Capsid-specific restriction factor that prevents infection from non-host-adapted retroviruses. Blocks viral replication early in the life cycle, after viral entry but before reverse transcription. In addition to acting as a capsid-specific restriction factor, also acts as a pattern recognition receptor that activates innate immune signaling in response to the retroviral capsid lattice. Binding to the viral capsid triggers its E3 ubiquitin ligase activity, and in concert with the heterodimeric ubiquitin conjugating enzyme complex UBE2V1-UBE2N (also known as UBC13-UEV1A complex) generates 'Lys-63'-linked polyubiquitin chains, which in turn are catalysts in the autophosphorylation of the MAP3K7/TAK1 complex (includes TAK1, TAB2, and TAB3). Activation of the MAP3K7/TAK1 complex by autophosphorylation results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes, thereby leading to an innate immune response in the infected cell. Restricts infection by human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency virus (SIV-agm).<ref>PMID:21734049</ref> <ref>PMID:21035162</ref> <ref>PMID:22291694</ref>
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[https://www.uniprot.org/uniprot/TRIM5_MACMU TRIM5_MACMU] Capsid-specific restriction factor that prevents infection from non-host-adapted retroviruses. Blocks viral replication early in the life cycle, after viral entry but before reverse transcription. In addition to acting as a capsid-specific restriction factor, also acts as a pattern recognition receptor that activates innate immune signaling in response to the retroviral capsid lattice. Binding to the viral capsid triggers its E3 ubiquitin ligase activity, and in concert with the heterodimeric ubiquitin conjugating enzyme complex UBE2V1-UBE2N (also known as UBC13-UEV1A complex) generates 'Lys-63'-linked polyubiquitin chains, which in turn are catalysts in the autophosphorylation of the MAP3K7/TAK1 complex (includes TAK1, TAB2, and TAB3). Activation of the MAP3K7/TAK1 complex by autophosphorylation results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes, thereby leading to an innate immune response in the infected cell. Restricts infection by human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency virus (SIV-agm).<ref>PMID:21734049</ref> <ref>PMID:21035162</ref> <ref>PMID:22291694</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Tripartite motif protein TRIM5alpha blocks retroviral replication after cell entry, and species-specific differences in its activity are determined by sequence variations within the C-terminal B30.2/PRYSPRY domain. Here we report a high-resolution structure of a TRIM5alpha PRYSPRY domain, the PRYSPRY of the rhesus monkey TRIM5alpha that potently restricts HIV infection, and identify features involved in its interaction with the HIV capsid. The extensive capsid-binding interface maps on the structurally divergent face of the protein formed by hypervariable loop segments, confirming that TRIM5alpha evolution is largely determined by its binding specificity. Interactions with the capsid are mediated by flexible variable loops via a mechanism that parallels antigen recognition by IgM antibodies, a similarity that may help explain some of the unusual functional properties of TRIM5alpha. Distinctive features of this pathogen-recognition interface, such as structural plasticity conferred by the mobile v1 segment and interaction with multiple epitopes, may allow restriction of divergent retroviruses and increase resistance to capsid mutations.
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Structure of the rhesus monkey TRIM5alpha PRYSPRY domain, the HIV capsid recognition module.,Biris N, Yang Y, Taylor AB, Tomashevski A, Guo M, Hart PJ, Diaz-Griffero F, Ivanov DN Proc Natl Acad Sci U S A. 2012 Aug 14;109(33):13278-83. doi:, 10.1073/pnas.1203536109. Epub 2012 Jul 30. PMID:22847415<ref>PMID:22847415</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3uv9" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
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</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Macmu]]
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[[Category: Macaca mulatta]]
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[[Category: Biris, N]]
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[[Category: Biris N]]
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[[Category: Diaz-Griffero, F]]
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[[Category: Diaz-Griffero F]]
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[[Category: Guo, M]]
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[[Category: Guo M]]
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[[Category: Hart, P J]]
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[[Category: Hart PJ]]
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[[Category: Ivanov, D]]
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[[Category: Ivanov D]]
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[[Category: Taylor, A B]]
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[[Category: Taylor AB]]
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[[Category: Tomashevskii, A]]
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[[Category: Tomashevskii A]]
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[[Category: Yang, Y]]
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[[Category: Yang Y]]
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[[Category: Antiretroviral]]
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[[Category: Domain swap]]
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[[Category: Hiv capsid]]
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[[Category: Ligase]]
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Current revision

Structure of the rhesus monkey TRIM5alpha deltav1 PRYSPRY domain

PDB ID 3uv9

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Proteopedia Page Contributors and Editors (what is this?)

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