1r3g

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[[Image:1r3g.gif|left|200px]]
[[Image:1r3g.gif|left|200px]]
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{{Structure
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|PDB= 1r3g |SIZE=350|CAPTION= <scene name='initialview01'>1r3g</scene>, resolution 1.16&Aring;
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The line below this paragraph, containing "STRUCTURE_1r3g", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=GMU:2&#39;-O-[(2-GUANIDINIUM)ETHYL]-5-METHYLURIDINE+5&#39;-MONOPHOSPHATE'>GMU</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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|GENE=
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|DOMAIN=
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{{STRUCTURE_1r3g| PDB=1r3g | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1r3g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r3g OCA], [http://www.ebi.ac.uk/pdbsum/1r3g PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1r3g RCSB]</span>
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'''1.16A X-ray structure of the synthetic DNA fragment with the incorporated 2'-O-[(2-Guanidinium)ethyl]-5-methyluridine residues'''
'''1.16A X-ray structure of the synthetic DNA fragment with the incorporated 2'-O-[(2-Guanidinium)ethyl]-5-methyluridine residues'''
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==About this Structure==
==About this Structure==
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1R3G is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R3G OCA].
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R3G OCA].
==Reference==
==Reference==
2'-O-[2-(guanidinium)ethyl]-modified oligonucleotides: stabilizing effect on duplex and triplex structures., Prakash TP, Puschl A, Lesnik E, Mohan V, Tereshko V, Egli M, Manoharan M, Org Lett. 2004 Jun 10;6(12):1971-4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15176796 15176796]
2'-O-[2-(guanidinium)ethyl]-modified oligonucleotides: stabilizing effect on duplex and triplex structures., Prakash TP, Puschl A, Lesnik E, Mohan V, Tereshko V, Egli M, Manoharan M, Org Lett. 2004 Jun 10;6(12):1971-4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15176796 15176796]
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[[Category: Protein complex]]
 
[[Category: Egli, M.]]
[[Category: Egli, M.]]
[[Category: Lesnik, E.]]
[[Category: Lesnik, E.]]
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[[Category: Puschl, A.]]
[[Category: Puschl, A.]]
[[Category: Tereshko, V.]]
[[Category: Tereshko, V.]]
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[[Category: dna]]
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[[Category: Dna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:01:45 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:22:41 2008''
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Revision as of 04:01, 3 May 2008

Image:1r3g.gif

Template:STRUCTURE 1r3g

1.16A X-ray structure of the synthetic DNA fragment with the incorporated 2'-O-[(2-Guanidinium)ethyl]-5-methyluridine residues


Overview

[structure: see text] Oligonucleotides with a novel 2'-O-[2-(guanidinium)ethyl] (2'-O-GE) modification have been synthesized using a novel protecting group strategy for the guanidinium group. This modification enhances the binding affinity of oligonucleotides to RNA as well as duplex DNA (DeltaT(m) 3.2 degrees C per modification). The 2'-O-GE modified oligonucleotides exhibited exceptional resistance to nuclease degradation. The crystal structure of a palindromic duplex formed by a DNA oligonucleotide with a single 2'-O-GE modification was solved at 1.16 A resolution.

About this Structure

Full crystallographic information is available from OCA.

Reference

2'-O-[2-(guanidinium)ethyl]-modified oligonucleotides: stabilizing effect on duplex and triplex structures., Prakash TP, Puschl A, Lesnik E, Mohan V, Tereshko V, Egli M, Manoharan M, Org Lett. 2004 Jun 10;6(12):1971-4. PMID:15176796 Page seeded by OCA on Sat May 3 07:01:45 2008

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