4eq5

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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4eq5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermococcus_sibiricus_MM_739 Thermococcus sibiricus MM 739]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EQ5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EQ5 FirstGlance]. <br>
<table><tr><td colspan='2'>[[4eq5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermococcus_sibiricus_MM_739 Thermococcus sibiricus MM 739]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EQ5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EQ5 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.85&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eq5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eq5 OCA], [https://pdbe.org/4eq5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eq5 RCSB], [https://www.ebi.ac.uk/pdbsum/4eq5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eq5 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eq5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eq5 OCA], [https://pdbe.org/4eq5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eq5 RCSB], [https://www.ebi.ac.uk/pdbsum/4eq5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eq5 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[https://www.uniprot.org/uniprot/C6A2U9_THESM C6A2U9_THESM]] DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.[HAMAP-Rule:MF_00407]
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[https://www.uniprot.org/uniprot/C6A2U9_THESM C6A2U9_THESM] DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.[HAMAP-Rule:MF_00407]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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DNA ligases join single-strand breaks in double-stranded DNA by catalyzing the formation of a phosphodiester bond between adjacent 5'-phosphate and 3'-hydroxyl termini. Their function is essential to maintain the integrity of the genome in DNA replication, recombination and repair. A recombinant ATP-dependent DNA ligase from the hyperthermophilic anaerobic archaeon Thermococcus sibiricus was expressed in Escherichia coli and purified. Crystals were grown by vapour diffusion using the hanging-drop method with 17%(w/v) PEG 4000 and 8.5%(v/v) 2-propanol as precipitants. A diffraction experiment was performed with a single crystal, which diffracted X-rays to 3.0 A resolution. The crystal belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 58.590, b = 87.540, c = 126.300 A.
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Expression, purification, crystallization and preliminary crystallographic analysis of a thermostable DNA ligase from the archaeon Thermococcus sibiricus.,Petrova TE, Bezsudnova EY, Dorokhov BD, Slutskaya ES, Polyakov KM, Dorovatovskiy PV, Ravin NV, Skryabin KG, Kovalchuk MV, Popov VO Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Feb 1;68(Pt 2):163-5. Epub, 2012 Jan 25. PMID:22297989<ref>PMID:22297989</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4eq5" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
*[[DNA ligase 3D structures|DNA ligase 3D structures]]
*[[DNA ligase 3D structures|DNA ligase 3D structures]]
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== References ==
 
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<references/>
 
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</StructureSection>
</StructureSection>

Current revision

DNA ligase from the archaeon Thermococcus sibiricus

PDB ID 4eq5

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