4evz

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PI:HYDROGENPHOSPHATE+ION'>PI</scene></td></tr>

<div style="background-color:#fffaf0;">

== Publication Abstract from PubMed ==

Due to the lack of macromolecular fossils, the enzymatic repertoire of extinct species has remained largely unknown to date. In an attempt to solve this problem, we have characterized a cyclase subunit (HisF) of the imidazole glycerol phosphate synthase (ImGP-S), which was reconstructed from the era of the last universal common ancestor of cellular organisms (LUCA). As observed for contemporary HisF proteins, the crystal structure of LUCA-HisF adopts the (betaalpha)8-barrel architecture, one of the most ancient folds. Moreover, LUCA-HisF (i) resembles extant HisF proteins with regard to internal 2-fold symmetry, active site residues, and a stabilizing salt bridge cluster, (ii) is thermostable and shows a folding mechanism similar to that of contemporary (betaalpha)8-barrel enzymes, (iii) displays high catalytic activity, and (iv) forms a stable and functional complex with the glutaminase subunit (HisH) of an extant ImGP-S. Furthermore, we show that LUCA-HisF binds to a reconstructed LUCA-HisH protein with high affinity. Our findings suggest that the evolution of highly efficient enzymes and enzyme complexes has already been completed in the LUCA era, which means that sophisticated catalytic concepts such as substrate channeling and allosteric communication existed already 3.5 billion years ago.

Evidence for the existence of elaborate enzyme complexes in the Paleoarchean era.,Reisinger B, Sperl J, Holinski A, Schmid V, Rajendran C, Carstensen L, Schlee S, Blanquart S, Merkl R, Sterner R J Am Chem Soc. 2014 Jan 8;136(1):122-9. doi: 10.1021/ja4115677. Epub 2013 Dec 23. PMID:24364418<ref>PMID:24364418</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

</div>

<div class="pdbe-citations 4evz" style="background-color:#fffaf0;"></div>

== References ==

<references/>