1thb

From Proteopedia

Revision as of 17:18, 12 November 2007

REFINEMENT OF A PARTIALLY OXYGENATED T STATE HAEMOGLOBIN AT 1.5 ANGSTROMS RESOLUTION

Overview

The degree of ligation of T state human haemoglobin crystals is reduced by, inositol hexaphosphate (IHP). The structure of a partially ligated, haemoglobin has been refined using fast Fourier restrained-least-squares, techniques. Manual interventions were required to escape from local minima, and introduce a large number of solvent molecules. Individual isotropic, temperature factors were refined for all atoms and the final average, atomic temperature factor is 32.3 A2. The final R factor is 19.6% for all, data between 10 and 1.5 A. The final model consists of 4560 protein atoms, and 313 solvent molecules. The occupancies of the ligand atoms and the, anisotropic behaviour of the iron atoms have been refined, demonstrating, that the alpha haem groups are only partially ligated and that there is no, ligation of the beta haems. Density for the IHP indicates that it is not, well ordered even though changes in the ligation and structure of the, haemoglobin indicate its presence.

Disease

Known diseases associated with this structure: Erythremias, alpha- OMIM:[141800], Erythremias, beta- OMIM:[141900], Erythrocytosis OMIM:[141850], HPFH, deletion type OMIM:[141900], Heinz body anemia OMIM:[141850], Heinz body anemias, alpha- OMIM:[141800], Heinz body anemias, beta- OMIM:[141900], Hemoglobin H disease OMIM:[141850], Hypochromic microcytic anemia OMIM:[141850], Methemoglobinemias, alpha- OMIM:[141800], Methemoglobinemias, beta- OMIM:[141900], Sickle cell anemia OMIM:[141900], Thalassemia, alpha- OMIM:[141850], Thalassemia-beta, dominant inclusion-body OMIM:[141900], Thalassemias, alpha- OMIM:[141800], Thalassemias, beta- OMIM:[141900]

About this Structure

1THB is a Protein complex structure of sequences from Homo sapiens with HEM, OXY and IHP as ligands. Full crystallographic information is available from OCA.

Reference

Refinement of a partially oxygenated T state human haemoglobin at 1.5 A resolution., Waller DA, Liddington RC, Acta Crystallogr B. 1990 Jun 1;46 ( Pt 3):409-18. PMID:2383372

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