1r5x

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[[Image:1r5x.gif|left|200px]]
[[Image:1r5x.gif|left|200px]]
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{{Structure
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|PDB= 1r5x |SIZE=350|CAPTION= <scene name='initialview01'>1r5x</scene>, resolution 2.30&Aring;
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The line below this paragraph, containing "STRUCTURE_1r5x", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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|GENE= AF2198 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224325 Archaeoglobus fulgidus DSM 4304])
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|DOMAIN=
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{{STRUCTURE_1r5x| PDB=1r5x | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1r5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r5x OCA], [http://www.ebi.ac.uk/pdbsum/1r5x PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1r5x RCSB]</span>
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'''JAMM: A Metalloprotease-like Zinc Site in the Proteasome and Signalosome'''
'''JAMM: A Metalloprotease-like Zinc Site in the Proteasome and Signalosome'''
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[[Category: Deshaies, R J.]]
[[Category: Deshaies, R J.]]
[[Category: Rees, D C.]]
[[Category: Rees, D C.]]
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[[Category: csn5]]
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[[Category: Csn5]]
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[[Category: jab1]]
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[[Category: Jab1]]
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[[Category: jamm]]
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[[Category: Jamm]]
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[[Category: mov34]]
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[[Category: Mov34]]
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[[Category: mpn]]
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[[Category: Mpn]]
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[[Category: proteasome]]
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[[Category: Proteasome]]
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[[Category: rpn11]]
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[[Category: Rpn11]]
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[[Category: signalosome]]
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[[Category: Signalosome]]
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[[Category: structural genomic]]
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[[Category: Structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:07:18 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:23:43 2008''
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Revision as of 04:07, 3 May 2008

Image:1r5x.gif

Template:STRUCTURE 1r5x

JAMM: A Metalloprotease-like Zinc Site in the Proteasome and Signalosome


Overview

The JAMM (JAB1/MPN/Mov34 metalloenzyme) motif in Rpn11 and Csn5 underlies isopeptidase activities intrinsic to the proteasome and signalosome, respectively. We show here that the archaebacterial protein AfJAMM possesses the key features of a zinc metalloprotease, yet with a distinct fold. The histidine and aspartic acid of the conserved EX(n)HS/THX(7)SXXD motif coordinate a zinc, whereas the glutamic acid hydrogen-bonds an aqua ligand. By analogy to the active site of thermolysin, we predict that the glutamic acid serves as an acid-base catalyst and the second serine stabilizes a tetrahedral intermediate. Mutagenesis of Csn5 confirms these residues are required for Nedd8 isopeptidase activity. The active site-like architecture specified by the JAMM motif motivates structure-based approaches to the study of JAMM domain proteins and the development of therapeutic proteasome and signalosome inhibitors.

About this Structure

1R5X is a Single protein structure of sequence from Archaeoglobus fulgidus dsm 4304. Full crystallographic information is available from OCA.

Reference

JAMM: a metalloprotease-like zinc site in the proteasome and signalosome., Ambroggio XI, Rees DC, Deshaies RJ, PLoS Biol. 2004 Jan;2(1):E2. Epub 2003 Nov 24. PMID:14737182 Page seeded by OCA on Sat May 3 07:07:18 2008

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