3gwh
From Proteopedia
(Difference between revisions)
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<StructureSection load='3gwh' size='340' side='right'caption='[[3gwh]], [[Resolution|resolution]] 1.95Å' scene=''> | <StructureSection load='3gwh' size='340' side='right'caption='[[3gwh]], [[Resolution|resolution]] 1.95Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[3gwh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/ | + | <table><tr><td colspan='2'>[[3gwh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GWH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GWH FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95Å</td></tr> |
| - | <tr id=' | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> |
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gwh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gwh OCA], [https://pdbe.org/3gwh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gwh RCSB], [https://www.ebi.ac.uk/pdbsum/3gwh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gwh ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gwh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gwh OCA], [https://pdbe.org/3gwh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gwh RCSB], [https://www.ebi.ac.uk/pdbsum/3gwh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gwh ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
| - | + | [https://www.uniprot.org/uniprot/GLCT_BACSU GLCT_BACSU] Mediates the positive regulation of the glucose PTS operon (ptsGHI) by functioning as an antiterminator factor of transcription via its interaction with the RNA-antiterminator (RAT) sequence located upstream of the ptsG gene. The RNA-binding domain of GlcT directly binds to the RNA antiterminator (RAT) sequence and prevents transcriptional termination. GlcT binding requires two identical and nearly symmetrical triple base pairings in the RAT sequence.<ref>PMID:11902727</ref> <ref>PMID:9765562</ref> | |
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3gwh ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3gwh ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | Ab initio macromolecular phasing has been so far limited to small proteins diffracting at atomic resolution (beyond 1.2 A) unless heavy atoms are present. We describe a general ab initio phasing method for 2 A data, based on combination of localizing model fragments such as small a-helices with Phaser and density modification with SHELXE. We implemented this approach in the program Arcimboldo to solve a 222-amino-acid structure at 1.95 A. | ||
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| - | Crystallographic ab initio protein structure solution below atomic resolution.,Rodriguez DD, Grosse C, Himmel S, Gonzalez C, de Ilarduya IM, Becker S, Sheldrick GM, Uson I Nat Methods. 2009 Sep;6(9):651-3. Epub 2009 Aug 16. PMID:19684596<ref>PMID:19684596</ref> | ||
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 3gwh" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Bacillus subtilis]] |
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Becker | + | [[Category: Becker S]] |
| - | [[Category: Gonzalez | + | [[Category: Gonzalez C]] |
| - | [[Category: Grosse | + | [[Category: Grosse C]] |
| - | [[Category: Himmel | + | [[Category: Himmel S]] |
| - | [[Category: Rodriguez | + | [[Category: Rodriguez DD]] |
| - | [[Category: Sheldrick | + | [[Category: Sheldrick GM]] |
| - | [[Category: Uson | + | [[Category: Uson I]] |
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Current revision
Crystallographic Ab Initio protein solution far below atomic resolution
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