1r64

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[[Image:1r64.jpg|left|200px]]
[[Image:1r64.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1r64 |SIZE=350|CAPTION= <scene name='initialview01'>1r64</scene>, resolution 2.20&Aring;
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The line below this paragraph, containing "STRUCTURE_1r64", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=BOR:(1R)-1-AMINO-4-{[(E)-AMINO(IMINO)METHYL]AMINO}BUTYLBORONIC+ACID'>BOR</scene>, <scene name='pdbligand=BTB:2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>BTB</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Kexin Kexin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.61 3.4.21.61] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= KEX2 OR QDS1 OR YNL238W OR N1122 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])
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-->
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|DOMAIN=
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{{STRUCTURE_1r64| PDB=1r64 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1r64 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r64 OCA], [http://www.ebi.ac.uk/pdbsum/1r64 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1r64 RCSB]</span>
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}}
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'''The 2.2 A crystal structure of Kex2 protease in complex with Ac-Arg-Glu-Lys-boroArg peptidyl boronic acid inhibitor'''
'''The 2.2 A crystal structure of Kex2 protease in complex with Ac-Arg-Glu-Lys-boroArg peptidyl boronic acid inhibitor'''
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[[Category: Petsko, G A.]]
[[Category: Petsko, G A.]]
[[Category: Ringe, D.]]
[[Category: Ringe, D.]]
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[[Category: kex2]]
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[[Category: Kex2]]
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[[Category: kexin]]
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[[Category: Kexin]]
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[[Category: prohormone processing]]
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[[Category: Prohormone processing]]
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[[Category: proprotein convertase]]
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[[Category: Proprotein convertase]]
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[[Category: protease]]
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[[Category: Protease]]
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[[Category: protein convertase]]
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[[Category: Protein convertase]]
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[[Category: subtilisin]]
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[[Category: Subtilisin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:07:45 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:23:47 2008''
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Revision as of 04:07, 3 May 2008

Image:1r64.jpg

Template:STRUCTURE 1r64

The 2.2 A crystal structure of Kex2 protease in complex with Ac-Arg-Glu-Lys-boroArg peptidyl boronic acid inhibitor


Overview

Kex2 is the yeast prototype of a large family of serine proteases that are highly specific for cleavage of their peptide substrates C-terminal to paired basic sites. This paper reports the 2.2 A resolution crystal structure of ssKex2 in complex with an Ac-Arg-Glu-Lys-Arg peptidyl boronic acid inhibitor (R = 19.7, R(free) = 23.4). By comparison of this structure with the structure of the mammalian homologue furin [Henrich, S., et al. (2003) Nat. Struct. Biol. 10, 520-526], we suggest a structural basis for the differences in substrate recognition at the P(2) and P(4) positions between Kex2 and furin and provide a structural rationale for the lack of P(6) recognition in Kex2. In addition, several monovalent cation binding sites are identified, and a mechanism of activation of Kex2 by potassium ion is proposed.

About this Structure

1R64 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Structural basis for differences in substrate selectivity in Kex2 and furin protein convertases., Holyoak T, Kettner CA, Petsko GA, Fuller RS, Ringe D, Biochemistry. 2004 Mar 9;43(9):2412-21. PMID:14992578 Page seeded by OCA on Sat May 3 07:07:45 2008

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