3u0j

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Current revision (08:38, 20 March 2024) (edit) (undo)
 
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<StructureSection load='3u0j' size='340' side='right'caption='[[3u0j]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
<StructureSection load='3u0j' size='340' side='right'caption='[[3u0j]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3u0j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Psesm Psesm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U0J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3U0J FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3u0j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_syringae_pv._tomato_str._DC3000 Pseudomonas syringae pv. tomato str. DC3000]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U0J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3U0J FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">hopU1, PSPTO_0501 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=223283 PSESM])</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3u0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u0j OCA], [https://pdbe.org/3u0j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3u0j RCSB], [https://www.ebi.ac.uk/pdbsum/3u0j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3u0j ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3u0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u0j OCA], [https://pdbe.org/3u0j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3u0j RCSB], [https://www.ebi.ac.uk/pdbsum/3u0j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3u0j ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q88A91_PSESM Q88A91_PSESM]
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The Pseudomonas syringae type III effector HopU1 is a mono-ADP-ribosyltransferase (mADP-RT) that is injected into plant cells by the type III protein secretion system. Inside the plant cell it suppresses immunity by modifying RNA-binding proteins including the glycine-rich RNA-binding protein GRP7. The crystal structure of HopU1 at 2.7 angstrom resolution reveals two unique protruding loops, L1 and L4 not found in other mADP-RTs. Site-directed mutagenesis demonstrates these loops are essential for substrate recognition and enzymatic activity. HopU1 ADP-ribosylates the conserved arginine 49 (R49) of GRP7 and this reduces GRP7's ability to bind RNA in vitro. In vivo, expression of GRP7 with R49 replaced with lysine does not complement the reduced immune responses of the Arabidopsis thaliana grp7-1 mutant demonstrating the importance of this residue for GRP7 function. These data provide mechanistic details how HopU1 recognizes this novel type of substrate and highlights the role of GRP7 in plant immunity.
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Structure function analysis of an ADP-ribosyltransferase type III effector and its RNA-binding target in plant immunity.,Jeong BR, Lin Y, Joe A, Guo M, Korneli C, Yang H, Wang P, Yu M, Cerny RL, Staiger D, Alfano JR, Xu Y J Biol Chem. 2011 Oct 19. PMID:22013065<ref>PMID:22013065</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3u0j" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Psesm]]
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[[Category: Pseudomonas syringae pv. tomato str. DC3000]]
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[[Category: Lin, Y]]
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[[Category: Lin Y]]
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[[Category: Wang, P]]
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[[Category: Wang P]]
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[[Category: Xu, Y]]
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[[Category: Xu Y]]
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[[Category: Yang, H]]
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[[Category: Yang H]]
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[[Category: Adp-ribosyltransferase]]
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[[Category: Grp7]]
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[[Category: Transferase]]
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Current revision

Crystal structure of ADP-ribosyltransferase HopU1 of Pseudomonas syringae pv. Tomato DC3000

PDB ID 3u0j

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