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| | <StructureSection load='3vol' size='340' side='right'caption='[[3vol]], [[Resolution|resolution]] 2.40Å' scene=''> | | <StructureSection load='3vol' size='340' side='right'caption='[[3vol]], [[Resolution|resolution]] 2.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3vol]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VOL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VOL FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3vol]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VOL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VOL FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CYN:CYANIDE+ION'>CYN</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.399Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">aer2, PA0176 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CYN:CYANIDE+ION'>CYN</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vol FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vol OCA], [https://pdbe.org/3vol PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vol RCSB], [https://www.ebi.ac.uk/pdbsum/3vol PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vol ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vol FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vol OCA], [https://pdbe.org/3vol PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vol RCSB], [https://www.ebi.ac.uk/pdbsum/3vol PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vol ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/MCPB_PSEAE MCPB_PSEAE] Chemoreceptor that plays a critical role in the virulence and pathogenesis of P.aeruginosa in a variety of hosts (PubMed:31511598). Probably acts through oxygen sensing (PubMed:31511598, PubMed:28167524, PubMed:34383467). Uses a heme-based sensor (PubMed:21255112, PubMed:22622145). Could be involved in chemotaxis (PubMed:12142407, PubMed:14987771). When expressed in E.coli, is able to sense and mediate repellent responses to oxygen, carbon monoxide and nitric oxide (PubMed:21255112).<ref>PMID:12142407</ref> <ref>PMID:14987771</ref> <ref>PMID:21255112</ref> <ref>PMID:22622145</ref> <ref>PMID:28167524</ref> <ref>PMID:31511598</ref> <ref>PMID:34383467</ref> |
| - | The crystal structure of a truncated Aer2, a signal transducer protein from Pseudomonas aeruginosa, consisting of the heme-containing PAS and di-HAMP domains revealed that a distal tryptophan residue (Trp283) plays an important role in stabilizing the heme-bound O(2) and intra-molecular signal transduction upon O(2) binding.
| + | |
| - | | + | |
| - | Structural basis for oxygen sensing and signal transduction of the heme-based sensor protein Aer2 from Pseudomonas aeruginosa.,Sawai H, Sugimoto H, Shiro Y, Ishikawa H, Mizutani Y, Aono S Chem Commun (Camb). 2012 Jul 4;48(52):6523-5. doi: 10.1039/c2cc32549g. Epub 2012 , May 23. PMID:22622145<ref>PMID:22622145</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div> | + | |
| - | <div class="pdbe-citations 3vol" style="background-color:#fffaf0;"></div> | + | |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Pseae]] | + | [[Category: Pseudomonas aeruginosa PAO1]] |
| - | [[Category: Aono, S]] | + | [[Category: Aono S]] |
| - | [[Category: Sawai, H]] | + | [[Category: Sawai H]] |
| - | [[Category: Shiro, Y]] | + | [[Category: Shiro Y]] |
| - | [[Category: Sugimoto, H]] | + | [[Category: Sugimoto H]] |
| - | [[Category: Cn-bound]]
| + | |
| - | [[Category: Cyanomet]]
| + | |
| - | [[Category: Hamp]]
| + | |
| - | [[Category: Heme]]
| + | |
| - | [[Category: Oxygen sensor protein]]
| + | |
| - | [[Category: Pa]]
| + | |
| - | [[Category: Signaling protein]]
| + | |
| Structural highlights
Function
MCPB_PSEAE Chemoreceptor that plays a critical role in the virulence and pathogenesis of P.aeruginosa in a variety of hosts (PubMed:31511598). Probably acts through oxygen sensing (PubMed:31511598, PubMed:28167524, PubMed:34383467). Uses a heme-based sensor (PubMed:21255112, PubMed:22622145). Could be involved in chemotaxis (PubMed:12142407, PubMed:14987771). When expressed in E.coli, is able to sense and mediate repellent responses to oxygen, carbon monoxide and nitric oxide (PubMed:21255112).[1] [2] [3] [4] [5] [6] [7]
References
- ↑ Ferrandez A, Hawkins AC, Summerfield DT, Harwood CS. Cluster II che genes from Pseudomonas aeruginosa are required for an optimal chemotactic response. J Bacteriol. 2002 Aug;184(16):4374-83. PMID:12142407
- ↑ Hong CS, Shitashiro M, Kuroda A, Ikeda T, Takiguchi N, Ohtake H, Kato J. Chemotaxis proteins and transducers for aerotaxis in Pseudomonas aeruginosa. FEMS Microbiol Lett. 2004 Feb 16;231(2):247-52. doi:, 10.1016/S0378-1097(04)00009-6. PMID:14987771 doi:http://dx.doi.org/10.1016/S0378-1097(04)00009-6
- ↑ Watts KJ, Taylor BL, Johnson MS. PAS/poly-HAMP signalling in Aer-2, a soluble haem-based sensor. Mol Microbiol. 2011 Feb;79(3):686-99. doi: 10.1111/j.1365-2958.2010.07477.x. Epub, 2010 Dec 7. PMID:21255112 doi:http://dx.doi.org/10.1111/j.1365-2958.2010.07477.x
- ↑ Sawai H, Sugimoto H, Shiro Y, Ishikawa H, Mizutani Y, Aono S. Structural basis for oxygen sensing and signal transduction of the heme-based sensor protein Aer2 from Pseudomonas aeruginosa. Chem Commun (Camb). 2012 Jul 4;48(52):6523-5. doi: 10.1039/c2cc32549g. Epub 2012 , May 23. PMID:22622145 doi:10.1039/c2cc32549g
- ↑ Garcia D, Orillard E, Johnson MS, Watts KJ. Gas Sensing and Signaling in the PAS-Heme Domain of the Pseudomonas aeruginosa Aer2 Receptor. J Bacteriol. 2017 Aug 22;199(18). pii: JB.00003-17. doi: 10.1128/JB.00003-17., Print 2017 Sep 15. PMID:28167524 doi:http://dx.doi.org/10.1128/JB.00003-17
- ↑ Garcia-Fontana C, Vilchez JI, Gonzalez-Requena M, Gonzalez-Lopez J, Krell T, Matilla MA, Manzanera M. The involvement of McpB chemoreceptor from Pseudomonas aeruginosa PAO1 in virulence. Sci Rep. 2019 Sep 11;9(1):13166. doi: 10.1038/s41598-019-49697-7. PMID:31511598 doi:http://dx.doi.org/10.1038/s41598-019-49697-7
- ↑ Orillard E, Anaya S, Johnson MS, Watts KJ. Oxygen-Induced Conformational Changes in the PAS-Heme Domain of the Pseudomonas aeruginosa Aer2 Receptor. Biochemistry. 2021 Aug 31;60(34):2610-2622. doi: 10.1021/acs.biochem.1c00452., Epub 2021 Aug 12. PMID:34383467 doi:http://dx.doi.org/10.1021/acs.biochem.1c00452
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